Overview

  • Product nameAnti-BHMT antibody [clone 3D6]
    See all BHMT primary antibodies
  • Description
    Mouse monoclonal [clone 3D6] to BHMT
  • Tested applicationsSuitable for: ELISA, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant human BHMT (1-406aa) purified from E. coli

  • Positive control
    • Extracts of mouse liver

Properties

Applications

Our Abpromise guarantee covers the use of ab52144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/1000 - 1/2000. Predicted molecular weight: 45 kDa.
Flow Cyt Use 0.1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionInvolved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
  • Tissue specificityFound exclusively in liver and kidney.
  • PathwayAmine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
    Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1.
  • Sequence similaritiesContains 1 Hcy-binding domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Betaine homocysteine methyltransferase antibody
    • Betaine homocysteine S methyltransferase 1 antibody
    • Betaine homocysteine S methyltransferase antibody
    • Betaine--homocysteine S-methyltransferase 1 antibody
    • BHMT antibody
    • BHMT1 antibody
    • BHMT1_HUMAN antibody
    • EC 2.1.1.5 antibody
    • Epididymis secretory sperm binding protein Li 61p antibody
    • HEL S 61p antibody
    see all

Anti-BHMT antibody [clone 3D6] images

  • Overlay histogram showing HepG2 cells stained with ab52144 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52144, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-BHMT antibody [clone 3D6] (ab52144) at 1/1000 dilution + extracts of mouse liver at 20 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

References for Anti-BHMT antibody [clone 3D6] (ab52144)

This product has been referenced in:
  • Li S  et al. Regulation of homocysteine homeostasis through the transcriptional coactivator PGC-1alpha. Am J Physiol Endocrinol Metab 296:E543-8 (2009). Read more (PubMed: 19158324) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"