Overview

  • Product name
  • Description
    Rabbit polyclonal to BHMT2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 94-363 of Human BHMT2.

  • Positive control
    • HCT116 and Mouse liver whole cell lysates; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab153990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. BHMT2 is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida.
  • Cellular localization
    Cytoplasmic
  • Database links
  • Alternative names
    • Betaine homocysteine methyltransferase 2 antibody
    • Betaine homocysteine S methyltransferase 2 antibody
    • EC 2.1.1.5 antibody
    • FLJ20001 antibody
    • S methylmethionine homocysteine S methyltransferase BHMT2 antibody
    • SMM hcy methyltransferase antibody
    see all

Images

  • Anti-BHMT2 antibody (ab153990) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Anti-BHMT2 antibody (ab153990) at 1/20000 dilution + Mouse liver whole cell lysate at 20 µg

    Predicted band size: 40 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling BHMT2 with ab153990 at 1/200 dilution. Lower panel co-stained with Hoechst 33342.

References

ab153990 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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