Overview

  • Product name
  • Description
    Rabbit polyclonal to BICD1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Frog
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 550-600 of Human BICD1 (NP_001705.2).

  • Positive control
    • Jurkat, 293T and HeLa whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab118802 was was affinity purified using an epitope specific to BICD1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab118802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 111 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
  • Tissue specificity
    Expressed in the brain, heart and skeletal muscle.
  • Sequence similarities
    Belongs to the BicD family.
  • Post-translational
    modifications
    Isoform 3 is phosphorylated on Ser-865 and Thr-866.
  • Cellular localization
    Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bic-D 1 antibody
    • Bicaudal D homolog 1 antibody
    • BICD antibody
    • Bicd1 antibody
    • BICD1_HUMAN antibody
    • Cytoskeleton like bicaudal D protein homolog 1 antibody
    • Protein bicaudal D homolog 1 antibody
    see all

Images

  • All lanes : Anti-BICD1 antibody (ab118802) at 0.1 µg/ml

    Lane 1 : Jurkat whole cell lysate at 50 µg
    Lane 2 : Jurkat whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 111 kDa


    Exposure time : 3 minutes
  • Detection of BICD1 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118802 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab118802 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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