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Rabbit polyclonal to BICD1
Predicted to work with:
Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus, Frog
Synthetic peptide corresponding to a region within amino acids 550-600 of Human BICD1 (NP_001705.2).
Jurkat, 293T and HeLa whole cell lysates.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab118802 was was affinity purified using an epitope specific to BICD1 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 111 kDa.
Use at 2-10 µg/mg of lysate.
Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
Expressed in the brain, heart and skeletal muscle.
Belongs to the BicD family.
Isoform 3 is phosphorylated on Ser-865 and Thr-866.
Information by UniProt
Bic-D 1 antibody
Bicaudal D homolog 1 antibody
Western blot - Anti-BICD1 antibody (ab118802)
All lanes : Anti-BICD1 antibody (ab118802) at 0.1 µg/ml Lane 1 : Jurkat whole cell lysate at 50 µg Lane 2 : Jurkat whole cell lysate at 15 µg Lane 3 : 293T whole cell lysate at 50 µg Lane 4 : HeLa whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 111 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-BICD1 antibody (ab118802)
Detection of BICD1 in Immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab118802 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"