Overview

  • Product name
  • Description
    Rabbit polyclonal to BICD2
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 375-425 of Human BICD2 (NP_056065.1).

  • Positive control
    • HeLa, 293T and Jurkat whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH: 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab117818 was affinity purified using an epitope specific to BICD2 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117818 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the BicD family.
  • Domain
    The fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2.
  • Post-translational
    modifications
    Phosphorylated by NEK9 in vitro.
  • Cellular localization
    Golgi apparatus. Cytoplasm > cytoskeleton. In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends (By similarity). Its localization is dependent on microtubule morphology.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA526D8.1 antibody
    • Bic D 2 antibody
    • Bic-D 2 antibody
    • Bicaudal D homolog 2 (Drosophila) antibody
    • Bicaudal D homolog 2 antibody
    • Bicd2 antibody
    • BICD2_HUMAN antibody
    • Coiled coil protein BICD2 antibody
    • Cytoskeleton like bicaudal D protein homolog 2 antibody
    • Homolog of Drosophila bicaudal D antibody
    • KIAA0699 antibody
    • Protein bicaudal D homolog 2 antibody
    see all

Images

  • All lanes : Anti-BICD2 antibody (ab117818) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 94 kDa


    Exposure time : 3 minutes
  • Detection of Human BICD2 by Immunoprecipitation in Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded) cells using ab117818 at 6 µg/mg of lysate. ab117818 at 0.4 µg/ml was used for subsequent WB detection of the immunoprecipitate.
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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