The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
FunctionMay play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
Sequence similaritiesBelongs to the BicD family.
DomainThe fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2.
Post-translational modificationsPhosphorylated by NEK9 in vitro.
Cellular localizationGolgi apparatus. Cytoplasm > cytoskeleton. In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends (By similarity). Its localization is dependent on microtubule morphology.