Overview

  • Product name
    Anti-BICD2 antibody - C-terminal
    See all BICD2 primary antibodies
  • Description
    Rabbit polyclonal to BICD2 - C-terminal
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the C terminal region of Human BICD2.

  • Positive control
    • K562 cell line lysates. Human hepatocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    ab80012 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab80012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/50 - 1/100. Predicted molecular weight: 93 kDa.
IHC-P 1/10 - 1/50.

Target

  • Function
    May play a role in the dynein-dynactin interactions on the surface of membranous organelles, by associating with these complexes. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the BicD family.
  • Domain
    The fourth coiled coil region is involved in Golgi targeting and in the interaction with DCTN2.
  • Post-translational
    modifications
    Phosphorylated by NEK9 in vitro.
  • Cellular localization
    Golgi apparatus. Cytoplasm > cytoskeleton. In interphase cells mainly localizes to the Golgi complex and colocalizes with dynactin at microtubule plus ends (By similarity). Its localization is dependent on microtubule morphology.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA526D8.1 antibody
    • Bic D 2 antibody
    • Bic-D 2 antibody
    • Bicaudal D homolog 2 (Drosophila) antibody
    • Bicaudal D homolog 2 antibody
    • Bicd2 antibody
    • BICD2_HUMAN antibody
    • Coiled coil protein BICD2 antibody
    • Cytoskeleton like bicaudal D protein homolog 2 antibody
    • Homolog of Drosophila bicaudal D antibody
    • KIAA0699 antibody
    • Protein bicaudal D homolog 2 antibody
    see all

Images

  • Anti-BICD2 antibody - C-terminal (ab80012) at 1/50 dilution + K562 cell line lysate at 35 µg

    Predicted band size : 93 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical staining of BICD2 in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using ab80012, at 1/10 dilution, and a peroxidase-conjugated secondary antibody and DAB staining.

References

ab80012 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab80012.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up