The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
1/50 - 1/100.
The major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
Isoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products. Phosphorylated upon DNA damage, probably by ATM or ATR. p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
Cytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
All lanes : Anti-Bid antibody (ab63541) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells (5ug) treated
with H2O2 (100uM, 30mins) Lane 2 : Extracts from Jurkat cells (5ug) treated with etoposide (25uM, 60 mins) Lane 3 : Extracts from Jurkat cells (5ug) treated
with H2O2 (100uM, 30mins) and etoposide (25uM, 60 mins) with immunizing non-phosphopeptide at 5 µg