Anti-Bid antibody - Carboxyterminal end (ab62469)

Overview

  • Product nameAnti-Bid antibody - Carboxyterminal end
    See all Bid primary antibodies
  • Description
    Rabbit polyclonal to Bid - Carboxyterminal end
  • SpecificityThis antibody reacts with Bid
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 14 amino acid synthetic peptide from near the carboxy-terminus of human Bid

  • Positive control
    • Mouse lung cell lysates and mouse lung tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 22 kDa).
IHC-P Use a concentration of 2 µg/ml.

Target

  • FunctionThe major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
  • Tissue specificityIsoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
  • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Post-translational
    modifications
    TNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation.
  • Cellular localizationCytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptic death agonist antibody
    • Apoptotic death agonist BID antibody
    • BH3 interacting domain death agonist antibody
    • BH3 interacting domain death agonist p11 antibody
    • BH3 interacting domain death agonist p13 antibody
    • BH3 interacting domain death agonist p15 antibody
    • BH3-interacting domain death agonist p11 antibody
    • BID antibody
    • BID isoform ES(1b) antibody
    • BID isoform L(2) antibody
    • BID isoform Si6 antibody
    • BID_HUMAN antibody
    • Desmocollin type 4 antibody
    • FP497 antibody
    • Human BID coding sequence antibody
    • MGC15319 antibody
    • MGC42355 antibody
    • p11 BID antibody
    • p13 BID antibody
    • p15 BID antibody
    • p22 BID antibody
    see all

Anti-Bid antibody - Carboxyterminal end images

  • Lane 1 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 0.5 µg/ml
    Lane 2 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 1 µg/ml
    Lane 3 : Anti-Bid antibody - Carboxyterminal end (ab62469) at 2 µg/ml

    Lane 1 : Mouse lung cell lysates
    Lane 2 : Mouse lung cell lysates
    Lane 3 : Mouse lung cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti rabbit IgG secondary antibody

    Predicted band size : 22 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical staining of mouse lung tissue using ab62469 antibody at 2 µg/ml.
  • Immunofluorescence of Bid in Mouse Lung cells using ab62469 at 10 ug/ml.

References for Anti-Bid antibody - Carboxyterminal end (ab62469)

ab62469 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MEF cell line)
Loading amount 30 µg
Specification MEF cell line
Treatment DMSO control and 2 µM SAHA for 24 hrs
Gel Running Conditions Reduced Denaturing (13%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

Submitted Feb 21 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HCT116 colon cancer cell line)
Loading amount 30 µg
Specification HCT116 colon cancer cell line
Treatment DMSO control and 2 µM SAHA for 24 hrs
Gel Running Conditions Reduced Denaturing (13%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Christian Marx

Verified customer

Submitted Feb 21 2013

Vielen Dank für Ihren Anruf.

Ich habe diesen Fall mit dem Labor besprochen. Leider konntenwirauch zusammenkeine Erklärung und somit Lösung dieses Problems mit diesem Röhrchen des ab62469 finden. Auch das Labor hat keine ne...

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Vielen Dank für Ihren Anruf.

Leider kann ich Ihnen die genaue Immunogensequenez nicht geben, ich kann Ihnen jedoch bestätigen, dass es eine Sequenz vom C Terminus ist, inzwischen den Aminosäuren 181 - 241.

Ich hoffe diese Informat...

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