Recombinant
Anti-Bid antibody [Y8] (ab32060)
- Datasheet
- Specific References (9)
- Protocols
Overview
- Product nameAnti-Bid antibody [Y8]
See all Bid primary antibodies - DescriptionRabbit monoclonal [Y8] to Bid
- SpecificityThis antibody does not cross-react with other Bcl-2 family members.
- Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details
- Species reactivityReacts with: Human
- Immunogen
A synthetic peptide corresponding to residues before the BH3 domain of human Bid.
- Positive control
- Jurkat cell lysate and human prostate carcinoma.
- General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Alternative versions available:
Anti-Bid antibody (HRP) [Y8] (ab201754)
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
-
Concentration information loading... - ClonalityMonoclonal
- Clone numberY8
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
- Positive Controls
- Related Products
Applications
Our Abpromise guarantee covers the use of ab32060 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | 1/100. | |
| Flow Cyt | 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| IP | 1/10. |
Target
- FunctionThe major proteolytic product p15 BID allows the release of cytochrome c (By similarity). Isoform 1, isoform 2 and isoform 4 induce ICE-like proteases and apoptosis. Isoform 3 does not induce apoptosis. Counters the protective effect of Bcl-2.
- Tissue specificityIsoform 2 and isoform 3 are expressed in spleen, bone marrow, cerebral and cerebellar cortex. Isoform 2 is expressed in spleen, pancreas and placenta (at protein level). Isoform 3 is expressed in lung, pancreas and spleen (at protein level). Isoform 4 is expressed in lung and pancreas (at protein level).
- DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
- Post-translational
modificationsTNF-alpha induces a caspase-mediated cleavage of p22 BID into a major p15 and minor p13 and p11 products.
Phosphorylated upon DNA damage, probably by ATM or ATR.
p15 BID is ubiquitinated by ITCH; ubiquitination results in proteasome-dependent degradation. - Cellular localizationCytoplasm; Cytoplasm. Mitochondrion membrane. When uncleaved, it is predominantly cytoplasmic; Mitochondrion membrane. A significant proportion of isoform 2 localizes to mitochondria, it may be cleaved constitutively; Mitochondrion membrane. Associated with the mitochondrial membrane and Mitochondrion membrane. Translocates to mitochondria as an integral membrane protein.
- Information by UniProt
-
Database links
- Entrez Gene: 637 Human
- Omim: 601997 Human
- SwissProt: P55957 Human
- Unigene: 591054 Human
-
Alternative names
- Apoptic death agonist antibody
- Apoptotic death agonist BID antibody
- BH3 interacting domain death agonist antibody
see all
Anti-Bid antibody [Y8] images
-
![Western blot - Anti-Bid antibody [Y8] (ab32060)](http://a.abcam.com/ps/products/32/ab32060/Images/ab32060wb.gif)
Western blot - Anti-Bid antibody [Y8] (ab32060)Anti-Bid antibody [Y8] (ab32060) at 1/1000 dilution + Jurkat cell lysate
Predicted band size : 22 kDa
Observed band size : 22 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody [Y8] (ab32060)](http://a.abcam.com/ps/products/32/ab32060/Images/ab32060ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bid antibody [Y8] (ab32060)Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using ab32060 at 1/100 dilution. -
![Western blot - Anti-Bid antibody [Y8] (ab32060)](http://a.abcam.com/ps/products/32/ab32060/Images/Bid-Primary-antibodies-ab32060-6.jpg)
Western blot - Anti-Bid antibody [Y8] (ab32060)Image courtesy of an anonymous Abreview.All lanes : Anti-Bid antibody [Y8] (ab32060) at 1/500 dilution
Lane 1 : Whole cell lysate prepared from breast cancer clinical extract
Lane 2 : Whole cell lysate prepared from breast cancer clinical extract
Lane 3 : Whole cell lysate prepared from MCF7 cells
Lysates/proteins at 20 µg per lane.
Secondary
Goat anti-rabbit IgG-HRP at 1/1000 dilution
Developed using the ECL technique
Predicted band size : 22 kDa
Observed band size : 22 kDa
Exposure time : 25 minutesImage courtesy of an anonymous Abreview.
-
![Flow Cytometry - Anti-Bid antibody [Y8] (ab32060)](http://a.abcam.com/ps/products/32/ab32060/Images/Bid-Primary-antibodies-ab32060-7.jpg)
Flow Cytometry - Anti-Bid antibody [Y8] (ab32060)Overlay histogram showing HeLa cells stained with ab32060 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32060, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-Bid antibody [Y8] (ab32060)
This product has been referenced in:
- Wang C et al. Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors. BMC Cancer 16:545 (2016). WB ; Human . Read more (PubMed: 27464624) »
- Hearn JM et al. Potent organo-osmium compound shifts metabolism in epithelial ovarian cancer cells. Proc Natl Acad Sci U S A 112:E3800-5 (2015). Human . Read more (PubMed: 26162681) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"