Overview

  • Product nameAnti-Bid Cleavage Site antibody
    See all Bid Cleavage Site primary antibodies
  • Description
    Rabbit polyclonal to Bid Cleavage Site
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Mouse)corresponding to N-terminus of cleavage site (59/60).

  • Positive control
    • TNF alpha treated L929 cells.
  • General notes


    BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.

Properties

Applications

Our Abpromise guarantee covers the use of ab10640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 15 kDa.

Target

  • RelevanceBid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
  • Database links
  • Alternative names
    • Apoptic death agonist antibody
    • BH 3 interacting domain death agonist antibody
    • BH3 interacting domain death agonist antibody
    • BID antibody
    • BID isoform ES(1b) antibody
    • BID isoform L(2) antibody
    • BID isoform Si6 antibody
    • Desmocollin type 4 antibody
    • FP497 antibody
    • Human BID coding sequence antibody
    • MGC15319 antibody
    • MGC42355 antibody
    • p22 BID antibody
    see all

Anti-Bid Cleavage Site antibody images



  • Predicted band size : 15 kDa


    Mouse L929 cells were treated with 5 µg/mL actinomycin plus 2.5 ng/mL, 5 ng/mL and 10 ng/mL mouse TNF alpha for 5 hours (Lane 3, 4 and 5). Untreated L929 cells (Lane 1) and cells treated with actinomycin alone (Lane 2) were used as controls. Proteins were resolved from the cells by SDS-PAGE. The proteins were transferred to PVDF membranes. The membranes were incubated with the anti-mouse full length BID antibody (left panel) and ab10640 (right panel) at 1 µg/mL. The signal was detected using a goat F(ab’)2 anti-rabbit IgG alkaline phosphatase antibody at a 1/5000 dilution and the membrane was incubated with CDP-substrate followed by chemiluminescence. The data show that ab10640 only recognizes the cleaved mouse BID and not full length (Right panel). The full length BID was revealed using an anti-mouse full length BID antibody (Left panel).

References for Anti-Bid Cleavage Site antibody (ab10640)

This product has been referenced in:
  • Qi Y  et al. Autophagy inhibition by sustained overproduction of IL6 contributes to arsenic carcinogenesis. Cancer Res 74:3740-52 (2014). Read more (PubMed: 24830721) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Breast)
Loading amount 20 µg
Specification Breast
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 16°C
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Abcam user community

Verified customer

Submitted Mar 18 2013

Thank you for your enquiry. The antiserum was produced against a chemically synthesized peptide derived from the N-terminus of cleavage site of mouse BID. Ab10640 recognizes the epitope which is created when BID is cleaved by caspase 8.Caspase -8 cl...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"