Recombinant
RabMAb

Anti-Bid Cleavage Site antibody [EPR9] (ab108293)

Overview

  • Product name
    Anti-Bid Cleavage Site antibody [EPR9]
    See all Bid Cleavage Site primary antibodies
  • Description
    Rabbit monoclonal [EPR9] to Bid Cleavage Site
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Bid Cleavage Site

  • Positive control
    • Jurkat, 293T and Human fetal brain lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Relevance
      Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
    • Database links
    • Alternative names
      • Apoptic death agonist antibody
      • BH 3 interacting domain death agonist antibody
      • BH3 interacting domain death agonist antibody
      • BID antibody
      • BID isoform ES(1b) antibody
      • BID isoform L(2) antibody
      • BID isoform Si6 antibody
      • Desmocollin type 4 antibody
      • FP497 antibody
      • Human BID coding sequence antibody
      • MGC15319 antibody
      • MGC42355 antibody
      • p22 BID antibody
      see all

    Images

    • All lanes : Anti-Bid Cleavage Site antibody [EPR9] (ab108293) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : 293T lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 22 kDa

    References

    ab108293 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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