Anti-Bid Cleavage Site antibody [EPR9] (ab108293)

Overview

  • Product nameAnti-Bid Cleavage Site antibody [EPR9]
    See all Bid Cleavage Site primary antibodies
  • Description
    Rabbit monoclonal [EPR9] to Bid Cleavage Site
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Bid Cleavage Site

  • Positive control
    • Jurkat, 293T and Human fetal brain lysates
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab108293 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
  • Application notesIs unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • RelevanceBid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas.
    • Database links
    • Alternative names
      • Apoptic death agonist antibody
      • BH 3 interacting domain death agonist antibody
      • BH3 interacting domain death agonist antibody
      • BID antibody
      • BID isoform ES(1b) antibody
      • BID isoform L(2) antibody
      • BID isoform Si6 antibody
      • Desmocollin type 4 antibody
      • FP497 antibody
      • Human BID coding sequence antibody
      • MGC15319 antibody
      • MGC42355 antibody
      • p22 BID antibody
      see all

    Anti-Bid Cleavage Site antibody [EPR9] images

    • All lanes : Anti-Bid Cleavage Site antibody [EPR9] (ab108293) at 1/1000 dilution

      Lane 1 : Jurkat lysate
      Lane 2 : 293T lysate
      Lane 3 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 22 kDa

    References for Anti-Bid Cleavage Site antibody [EPR9] (ab108293)

    ab108293 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"