Overview

  • Product nameAnti-Bif antibody [1B3-A5]
    See all Bif primary antibodies
  • Description
    Mouse monoclonal [1B3-A5] to Bif
  • Tested applicationsSuitable for: Sandwich ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human Bif with proprietary tag (AAH07455, 1 aa - 365 aa).

  • Positive control
    • Human Spleen and Testis tissue; K-562 cell lysate; Bif transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab110091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged Bif is approximately 0.3 ng/ml as a capture antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 41 kDa.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • FunctionMay be required for normal outer mitochondrial membrane dynamics (PubMed:15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability.
  • Tissue specificityHighly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the endophilin family.
    Contains 1 BAR domain.
    Contains 1 SH3 domain.
  • DomainAn N-terminal amphipathic helix, the BAR domain and a second amphipathic helix inserted into helix 1 of the BAR domain (N-BAR domain) induce membrane curvature and bind curved membranes.
    The SH3 domain is required and sufficient for the interaction with UVRAG.
  • Post-translational
    modifications
    Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization.
  • Cellular localizationCytoplasm. Golgi apparatus membrane. Mitochondrion outer membrane. Cytoplasmic vesicle, autophagosome membrane. Midbody. Association with the Golgi apparatus depends on the cell type (By similarity). Following starvation colocalizes with ATG5 and LC3 autophagy-related protein(s)on autophagosomal membranes (PubMed:17891140).
  • Information by UniProt
  • Database links
  • Alternative names
    • Bax interacting factor 1 antibody
    • Bax-interacting factor 1 antibody
    • Bif 1 antibody
    • Bif-1 antibody
    • CGI 61 antibody
    • dJ612B15.2 antibody
    • Endophilin B1 antibody
    • Endophilin-B1 antibody
    • PPP1R70 antibody
    • Protein phosphatase 1 regulatory subunit 70 antibody
    • SH3 containing protein SH3GLB1 antibody
    • SH3 domain GRB2 like endophilin B1 antibody
    • SH3 domain-containing GRB2-like protein B1 antibody
    • Sh3glb1 antibody
    • SH3p13 antibody
    • SHLB1_HUMAN antibody
    see all

Anti-Bif antibody [1B3-A5] images

  • ab110091, at 2.5µg/ml, staining Bif in formalin-fixed, paraffin-embedded Human Spleen tissue by Immunohistochemistry.
  • ab110091, at 2.5µg/ml, staining Bif in formalin-fixed, paraffin-embedded Human Testis tissue by Immunohistochemistry.
  • All lanes : Anti-Bif antibody [1B3-A5] (ab110091)

    Lane 1 : Bif transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate


    Predicted band size : 41 kDa
  • Anti-Bif antibody [1B3-A5] (ab110091) + K-562 cell lysate

    Predicted band size : 41 kDa
  • Detection limit for recombinant tagged Bif is approximately 0.3 ng/ml as a capture antibody.

References for Anti-Bif antibody [1B3-A5] (ab110091)

ab110091 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"