• Product nameAnti-BIG1 antibody
    See all BIG1 primary antibodies
  • Description
    Rabbit polyclonal to BIG1
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1800 and the C-terminus (residue 1849) of human BIG1 (according to NP_006412.2)

  • Positive control
    • Whole cell lysate from HeLa, 293T or NIH3T3 cells.



Our Abpromise guarantee covers the use of ab72061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 208 kDa).
IP Use at 2-5 µg/mg of lysate.


  • RelevanceBIG1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
  • Cellular localizationCytoplasmic, Golgi Apparatus and Nuclear
  • Database links
  • Alternative names
    • ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited) antibody
    • ADP-ribosylation factor guanine nucleotide exchange factor 1 antibody
    • ARF GEP1 antibody
    • ARFGEF1 antibody
    • ARFGEP1 antibody
    • Brefeldin A inhibited GEP 1 antibody
    • Brefeldin A inhibited guanine nucleotide exchange protein 1 antibody
    • P200 antibody
    • p200 ARF guanine nucleotide exchange factor antibody
    see all

Anti-BIG1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BIG1 with ab72061 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-BIG1 antibody (ab72061) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg

    developed using the ECL technique

    Predicted band size : 208 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa,41 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human BIG1 by Immunoprecipitation in 1mg whole cell lysate from HeLa cells, using ab72061 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). Subsequent Western blot detection was performed using ab72061 at 1 µg/ml on 20% of the imunoprecipitate.

References for Anti-BIG1 antibody (ab72061)

ab72061 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (MDBK)
Loading amount 20 µg
Specification MDBK
Gel Running Conditions Reduced Denaturing (10% Tris-Glycine gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Submitted Oct 19 2012