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Rabbit polyclonal to BIG1
Predicted to work with:
Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to a region between residue 1800 and the C-terminus (residue 1849) of human BIG1 (according to NP_006412.2)
Whole cell lysate from HeLa, 293T or NIH3T3 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 208 kDa).
Use at 2-5 µg/mg of lysate.
BIG1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
Cytoplasmic, Golgi Apparatus and Nuclear
ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited) antibody
ADP-ribosylation factor guanine nucleotide exchange factor 1 antibody
ARF GEP1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIG1 antibody (ab72061)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BIG1 with ab72061 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - BIG1 antibody (ab72061)
All lanes :
Anti-BIG1 antibody (ab72061) at 0.1 µg/ml
Lane 1 :
cell lysate from HeLa cells at 50 µg
Lane 2 :
cell lysate from HeLa cells at 15 µg
Lane 3 :
cell lysate from HeLa cells at 5 µg
Lane 4 :
cell lysate from 293T cells at 50 µg
Lane 5 :
cell lysate from NIH 3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size:
Observed band size:
250 kDa (
why is the actual band size different from the predicted?
Additional bands at:
31 kDa, 41 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - BIG1 antibody (ab72061)
Detection of Human BIG1 by Immunoprecipitation in 1mg whole
cell lysate from HeLa cells, using ab72061 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). Subsequent Western blot detection was performed using ab72061 at 1 µg/ml on 20% of the imunoprecipitate.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"