Overview

  • Product nameAnti-BIG1 antibody
    See all BIG1 primary antibodies
  • Description
    Rabbit polyclonal to BIG1
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residue 1800 and the C-terminus (residue 1849) of human BIG1 (according to NP_006412.2)

  • Positive control
    • Whole cell lysate from HeLa, 293T or NIH3T3 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 250 kDa (predicted molecular weight: 208 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceBIG1 is a brefeldin A-inhibited guanine nucleotide exchange protein (GEP) that activates ADP-ribosylation factor (ARF) GTPases and functions in vesicle formation from Golgi membranes.
  • Cellular localizationCytoplasmic, Golgi Apparatus and Nuclear
  • Database links
  • Alternative names
    • ADP ribosylation factor guanine nucleotide exchange factor 1(brefeldin A inhibited) antibody
    • ADP-ribosylation factor guanine nucleotide exchange factor 1 antibody
    • ARF GEP1 antibody
    • ARFGEF1 antibody
    • ARFGEP1 antibody
    • Brefeldin A inhibited GEP 1 antibody
    • Brefeldin A inhibited guanine nucleotide exchange protein 1 antibody
    • P200 antibody
    • p200 ARF guanine nucleotide exchange factor antibody
    see all

Anti-BIG1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BIG1 with ab72061 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-BIG1 antibody (ab72061) at 0.1 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg
    Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 208 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa,41 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human BIG1 by Immunoprecipitation in 1mg whole cell lysate from HeLa cells, using ab72061 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). Subsequent Western blot detection was performed using ab72061 at 1 µg/ml on 20% of the imunoprecipitate.

References for Anti-BIG1 antibody (ab72061)

ab72061 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (MDBK)
Loading amount 20 µg
Specification MDBK
Gel Running Conditions Reduced Denaturing (10% Tris-Glycine gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"