Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BIG1 with ab72061 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - BIG1 antibody (ab72061)
All lanes : Anti-BIG1 antibody (ab72061) at 0.1 µg/ml
Lane 1 : Whole
cell lysate from HeLa cells at 50 µg Lane 2 : Whole
cell lysate from HeLa cells at 15 µg Lane 3 : Whole
cell lysate from HeLa cells at 5 µg Lane 4 : Whole
cell lysate from 293T cells at 50 µg Lane 5 : Whole
cell lysate from NIH 3T3 cells at 50 µg
Detection of Human BIG1 by Immunoprecipitation in 1mg whole
cell lysate from HeLa cells, using ab72061 at 3 µg/mg lysate (lane 1) or a control rabbit IgG (lane 2). Subsequent Western blot detection was performed using ab72061 at 1 µg/ml on 20% of the imunoprecipitate.