Overview

  • Product nameAnti-Biglycan antibody
    See all Biglycan primary antibodies
  • Description
    Rabbit polyclonal to Biglycan
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 216-265 (SEAKLTGIPK DLPETLNELH LDHNKIQAIE LEDLLRYSKL YRLGLGHNQI) of Human Biglycan (NP_001702).

  • Positive control
    • Human fetal heart lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab94460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay be involved in collagen fiber assembly.
  • Tissue specificityFound in several connective tissues, especially in articular cartilages.
  • Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
    Contains 12 LRR (leucine-rich) repeats.
  • Post-translational
    modifications
    The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
  • Cellular localizationSecreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • BGN antibody
    • Biglycan antibody
    • Biglycan proteoglycan antibody
    • Bone/cartilage proteoglycan I antibody
    • Dermatan sulphate proteoglycan I antibody
    • DSPG1 antibody
    • PG S1 antibody
    • PG-S1 antibody
    • PGI antibody
    • PGS1_HUMAN antibody
    • SLRR1A antibody
    • Small leucine rich protein 1A antibody
    see all

Anti-Biglycan antibody images

  • Anti-Biglycan antibody (ab94460) at 1 µg/ml + Human fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa

References for Anti-Biglycan antibody (ab94460)

ab94460 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Tissue lysate - whole (Aorta)
Specification Aorta
Treatment Non deglycosylated - Deglycosylated (PNGaseF)
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Feb 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"