Recombinant
RabMAb

Anti-Biglycan antibody [EPR2959] (ab109369)

Overview

  • Product name
    Anti-Biglycan antibody [EPR2959]
    See all Biglycan primary antibodies
  • Description
    Rabbit monoclonal [EPR2959] to Biglycan
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human Biglycan.

  • Positive control
    • Rat cartilage, C2C12, Human skeletal muscle and Mouse muscle lysates; L6 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function
      May be involved in collagen fiber assembly.
    • Tissue specificity
      Found in several connective tissues, especially in articular cartilages.
    • Sequence similarities
      Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
      Contains 12 LRR (leucine-rich) repeats.
    • Post-translational
      modifications
      The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • BGN antibody
      • Biglycan antibody
      • Biglycan proteoglycan antibody
      • Bone/cartilage proteoglycan I antibody
      • Dermatan sulphate proteoglycan I antibody
      • DSPG1 antibody
      • PG S1 antibody
      • PG-S1 antibody
      • PGI antibody
      • PGS1_HUMAN antibody
      • SEMDX antibody
      • SLRR1A antibody
      • Small leucine rich protein 1A antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized L6 cells using ab109369 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
    • All lanes : Anti-Biglycan antibody [EPR2959] (ab109369) at 1/1000 dilution

      Lane 1 : Rat cartilage lysate
      Lane 2 : L6 lysate
      Lane 3 : C2C12 lysate
      Lane 4 : Human skeletal muscle lysate
      Lane 5 : Mouse muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 42 kDa

    References

    This product has been referenced in:
    • Liu S  et al. Tyrosine kinase LYN is an oncotarget in human cervical cancer: A quantitative proteomic based study. Oncotarget 7:75468-75481 (2016). WB ; Human . Read more (PubMed: 27690342) »
    • Tan HT  et al. Unravelling the proteome of degenerative human mitral valves. Proteomics 15:2934-44 (2015). WB ; Human . Read more (PubMed: 25914152) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Zebrafish Tissue sections (Skeleton)
    Permeabilization
    No
    Specification
    Skeleton
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Mar 12 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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