Anti-Biglycan antibody [EPR2959] (ab109369)

Overview

  • Product nameAnti-Biglycan antibody [EPR2959]
    See all Biglycan primary antibodies
  • Description
    Rabbit monoclonal [EPR2959] to Biglycan
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Human Biglycan.

  • Positive control
    • Rat cartilage, C2C12, Human skeletal muscle and Mouse muscle lysates; L6 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 42 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionMay be involved in collagen fiber assembly.
    • Tissue specificityFound in several connective tissues, especially in articular cartilages.
    • Sequence similaritiesBelongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.
      Contains 12 LRR (leucine-rich) repeats.
    • Post-translational
      modifications
      The two attached glycosaminoglycan chains can be either chondroitin sulfate or dermatan sulfate.
    • Cellular localizationSecreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links
    • Alternative names
      • BGN antibody
      • Biglycan antibody
      • Biglycan proteoglycan antibody
      • Bone/cartilage proteoglycan I antibody
      • Dermatan sulphate proteoglycan I antibody
      • DSPG1 antibody
      • PG S1 antibody
      • PG-S1 antibody
      • PGI antibody
      • PGS1_HUMAN antibody
      • SLRR1A antibody
      • Small leucine rich protein 1A antibody
      see all

    Anti-Biglycan antibody [EPR2959] images

    • All lanes : Anti-Biglycan antibody [EPR2959] (ab109369) at 1/1000 dilution

      Lane 1 : Rat cartilage lysate
      Lane 2 : L6 lysate
      Lane 3 : C2C12 lysate
      Lane 4 : Human skeletal muscle lysate
      Lane 5 : Mouse muscle lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 42 kDa
    • Flow cytometric analysis of permeabilized L6 cells using ab109369 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

    References for Anti-Biglycan antibody [EPR2959] (ab109369)

    ab109369 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Zebrafish Tissue sections (Skeleton)
    Permeabilization No
    Specification Skeleton
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative Paraformaldehyde
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    Verified customer

    Submitted Mar 12 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"