Overview

  • Product nameAnti-Bik antibody
    See all Bik primary antibodies
  • Description
    Rabbit polyclonal to Bik
  • SpecificityDetects endogenous levels of total BIK protein.
  • Tested applicationsSuitable for: IHC-P, WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic non-phosphopeptide (human) around the phosphorylation site of threonine 33 (G-M-T-D-S).

  • Positive control
    • Extracts from A549 cells treated with DMSO, human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab52182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 18 kDa.
ELISA 1/10000.
ICC/IF Use a concentration of 1 - 5 µg/ml.

Target

  • FunctionAccelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
  • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localizationEndomembrane system. Around the nuclear envelope, and in cytoplasmic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis inducer NBK antibody
    • BBC1 antibody
    • Bcl-2-interacting killer antibody
    • BCL2 interacting killer antibody
    • bhikhari antibody
    • BIK antibody
    • Bik-like killer protein antibody
    • BIK_HUMAN antibody
    • BIP 1 antibody
    • BiP1 antibody
    • BP 4 antibody
    • BP4 antibody
    • cb 60 antibody
    • NBK antibody
    see all

Anti-Bik antibody images

  • All lanes : Anti-Bik antibody (ab52182) at 1/500 dilution

    Lane 1 : extracts from A549 cells, treated with DMSO (0.1%, 10mins)
    Lane 2 : extracts from A549 cells, treated with DMSO (0.1%, 10mins) with blocking peptide


    Predicted band size : 18 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Ab52182 at 1/50 dilution staining Human breast carcinoma tissue, with and without blocking peptide; paraffin embedded.
  • ICC/IF image of ab52182 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52182, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab52182 staining Bik in human Jurkat cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X and then blocked using 3% serum for 3 hours at 23°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 647 (pink) used at a 1/1000 dilution.

    See Abreview

References for Anti-Bik antibody (ab52182)

This product has been referenced in:
  • Wang X  et al. Synergistic control of sex hormones by 17ß-HSD type 7: a novel target for estrogen-dependent breast cancer. J Mol Cell Biol 7:568-79 (2015). Read more (PubMed: 25966904) »
  • Wu J  et al. Silencing of Kv1.5 Gene Inhibits Proliferation and Induces Apoptosis of Osteosarcoma Cells. Int J Mol Sci 16:26914-26 (2015). WB ; Human . Read more (PubMed: 26569226) »

See all 4 Publications for this product

Product Wall

Abreviews
Application Western blot
Sample Mouse Cell lysate - other (MEF)
Loading amount 50 µg
Specification MEF
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted Feb 24 2011

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat T lymphocytes)
Loading amount 50 µg
Specification Jurkat T lymphocytes
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3%
Username

Abcam user community

Verified customer

Submitted Feb 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cultured Cells (Jurkat)
Specification Jurkat
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Feb 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"