Anti-Bik antibody - Aminoterminal end (ab62494)

Overview

  • Product name
    Anti-Bik antibody - Aminoterminal end
    See all Bik primary antibodies
  • Description
    Rabbit polyclonal to Bik - Aminoterminal end
  • Specificity
    This antibody reacts with Bik
  • Tested applications
    Suitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A 15 amino acid synthetic peptide from near the amino terminus of human Bik

  • Positive control
    • Jurkat cell lysate and Jurkat cells

Properties

Applications

Our Abpromise guarantee covers the use of ab62494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa).
ICC Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
  • Domain
    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localization
    Endomembrane system. Around the nuclear envelope, and in cytoplasmic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis inducer NBK antibody
    • BBC1 antibody
    • Bcl-2-interacting killer antibody
    • BCL2 interacting killer antibody
    • bhikhari antibody
    • BIK antibody
    • Bik-like killer protein antibody
    • BIK_HUMAN antibody
    • BIP 1 antibody
    • BiP1 antibody
    • BP 4 antibody
    • BP4 antibody
    • cb 60 antibody
    • NBK antibody
    see all

Images

  • Lane 1 : Anti-Bik antibody - Aminoterminal end (ab62494) at 1 µg/ml
    Lane 2 : Anti-Bik antibody - Aminoterminal end (ab62494) at 2 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 18 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry of Bik in Jurkat cells with ab62494 antibody at 1 µg/ml.
  • Immunofluorescence of Bik in Jurkat cells using ab62494 at 10 ug/ml.

References

ab62494 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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