Overview

  • Product nameAnti-Bik (phospho T33) antibody
    See all Bik primary antibodies
  • Description
    Rabbit polyclonal to Bik (phospho T33)
  • Specificityab55179 detects endogenous levels of BIK only when phosphorylated at threonine 33.
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from human BIK around the phosphorylation site of threonine 33 (G-M-TP-D-S).

  • Positive control
    • human lung carcinoma tissue, extracts from HepG2 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab55179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 18 kDa).

Target

  • FunctionAccelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
  • DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localizationEndomembrane system. Around the nuclear envelope, and in cytoplasmic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis inducer NBK antibody
    • BBC1 antibody
    • Bcl-2-interacting killer antibody
    • BCL2 interacting killer antibody
    • bhikhari antibody
    • BIK antibody
    • Bik-like killer protein antibody
    • BIK_HUMAN antibody
    • BIP 1 antibody
    • BiP1 antibody
    • BP 4 antibody
    • BP4 antibody
    • cb 60 antibody
    • NBK antibody
    see all

Anti-Bik (phospho T33) antibody images

  • All lanes : Anti-Bik (phospho T33) antibody (ab55179) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells.
    Lane 2 : extracts from HepG2 cells, incubated with the immunising phosphopeptide.


    Predicted band size : 18 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of Bik (phospho T33) expression in paraffin embedded human lung carcinoma tissue using ab55179 at 1/50 dilution (left). Rightpanel represents a negative control where ab55179 was pre-incubated with the immunising (blocking) phosphopeptide.

References for Anti-Bik (phospho T33) antibody (ab55179)

ab55179 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"