The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 18 kDa).
FunctionAccelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
DomainIntact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localizationEndomembrane system. Around the nuclear envelope, and in cytoplasmic membranes.
Immunohistochemical analysis of Bik (phospho T33) expression in paraffin embedded human lung carcinoma tissue using ab55179 at 1/50 dilution (left). Rightpanel represents a negative control where ab55179 was pre-incubated with the immunising (blocking) phosphopeptide.
References for Anti-Bik (phospho T33) antibody (ab55179)
has not yet been referenced specifically in any publications.
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