Overview

  • Product name
  • Description
    Rabbit polyclonal to Bikunin
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

Properties

Applications

Our Abpromise guarantee covers the use of ab43073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 39 kDa.
ELISA 1/2000.

Target

  • Function
    Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization.
    Trypstatin is a trypsin inhibitor.
  • Tissue specificity
    Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
  • Sequence similarities
    In the N-terminal section; belongs to the calycin superfamily. Lipocalin family.
    Contains 2 BPTI/Kunitz inhibitor domains.
  • Post-translational
    modifications
    The precursor is proteolytically processed into separately functioning proteins.
    3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'.
    Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate.
    Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Form
    Bikunin, the light chain of the proteinase inhibitors inter-alpha-inhibitor and pre-alpha-inhibitor, is synthesized in the liver. Its biological function is unclear but it has been shown to inhibit proteases and to have additional activities in vitro. Bikunin is translated as a precursor protein from the same mRNA as alpha 1-microglobulin. This cosynthesis is unique compared to other proproteins since they have no known functional connection.
  • Alternative names
    • Alpha 1 microglobulin/bikunin precursor antibody
    • Alpha-1 microglycoprotein antibody
    • AMBP antibody
    • AMBP_HUMAN antibody
    • Bikunin antibody
    • Complex-forming glycoprotein heterogeneous in charge antibody
    • EDC1 antibody
    • Growth inhibiting protein 19 antibody
    • HI 30 antibody
    • HI-30 antibody
    • HI30 antibody
    • IATIL antibody
    • Inter alpha trypsin inhibitor light chain antibody
    • ITI antibody
    • ITI LC antibody
    • ITI-LC antibody
    • ITILC antibody
    • Protein HC antibody
    • Trypstatin antibody
    • Uronic-acid-rich protein antibody
    • UTI antibody
    see all

References for Anti-Bikunin antibody (ab43073)

This product has been referenced in:
  • Farrugia BL  et al. Mast Cells Produce a Unique Chondroitin Sulfate Epitope. J Histochem Cytochem 64:85-98 (2016). Read more (PubMed: 26586669) »
  • He H  et al. Immobilized heavy chain-hyaluronic acid polarizes lipopolysaccharide-activated macrophages toward M2 phenotype. J Biol Chem 288:25792-803 (2013). Read more (PubMed: 23878196) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us. Please fiind the MSDS documents for these antibodies attached. Please let me know if you need any additional informaiton.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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