The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/1000. Predicted molecular weight: 39 kDa.
Inter-alpha-trypsin inhibitor inhibits trypsin, plasmin, and lysosomal granulocytic elastase. Inhibits calcium oxalate crystallization. Trypstatin is a trypsin inhibitor.
Expressed by the liver and secreted in plasma. Alpha-1-microglobulin occurs in many physiological fluids including plasma, urine, and cerebrospinal fluid. Inter-alpha-trypsin inhibitor is present in plasma and urine.
In the N-terminal section; belongs to the calycin superfamily. Lipocalin family. Contains 2 BPTI/Kunitz inhibitor domains.
The precursor is proteolytically processed into separately functioning proteins. 3-hydroxykynurenine, an oxidized tryptophan metabolite that is common in biological fluids, reacts with Cys-53, Lys-111, Lys-137, and Lys-149 to form heterogeneous polycyclic chromophores including hydroxanthommatin. The reaction by alpha-1-microglobulin is autocatalytic; the human protein forms chromophore even when expressed in insect and bacterial cells. The chromophore can react with accessible cysteines forming non-reducible thioether cross-links with other molecules of alpha-1-microglobulin or with other proteins such as Ig alpha-1 chain C region 'Cys-352'. Heavy chains are interlinked with bikunin via a chondroitin 4-sulfate bridge to the their C-terminal aspartate. Addition of glycosaminoglycan chondroitin sulfate, allows cross-linking between the different components.
Bikunin, the light chain of the proteinase inhibitors inter-alpha-inhibitor and pre-alpha-inhibitor, is synthesized in the liver. Its biological function is unclear but it has been shown to inhibit proteases and to have additional activities in vitro. Bikunin is translated as a precursor protein from the same mRNA as alpha 1-microglobulin. This cosynthesis is unique compared to other proproteins since they have no known functional connection.
Alpha 1 microglobulin/bikunin precursor antibody
Alpha-1 microglycoprotein antibody
Complex-forming glycoprotein heterogeneous in charge antibody
Growth inhibiting protein 19 antibody
HI 30 antibody
Inter alpha trypsin inhibitor light chain antibody
ITI LC antibody
Protein HC antibody
Uronic-acid-rich protein antibody
Western blot - Anti-Bikunin antibody - N-terminal (ab189431)
Anti-Bikunin antibody - N-terminal (ab189431) at 1/1000 dilution + HepG2 cell lysate at 35 µg