Anti-Bile Acid Receptor NR1H4 antibody (ab58559)

Overview

  • Product nameAnti-Bile Acid Receptor NR1H4 antibody
    See all Bile Acid Receptor NR1H4 primary antibodies
  • Description
    Goat polyclonal to Bile Acid Receptor NR1H4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mouse, Cow, Syrian Hamster
  • Immunogen

    Synthetic peptide:

    KSCREKTELTPDQQ

    , corresponding to internal sequence amino acids 251-264 of Human Bile Acid Receptor NR1H4, (NP_005114.1.).

  • Positive control
    • Human Lung and Duodenal lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab58559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesWB: Use at a concentration of 1 - 3 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

    Peptide ELISA: antibody detection limit dilution 1:32,000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionLigand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.
    • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BAR antibody
      • Bile acid receptor antibody
      • Farnesoid X activated receptor antibody
      • Farnesoid X receptor antibody
      • Farnesoid X-activated receptor antibody
      • Farnesol receptor HRR 1 antibody
      • Farnesol receptor HRR-1 antibody
      • Farnesol receptor HRR1 antibody
      • FXR antibody
      • HRR 1 antibody
      • HRR1 antibody
      • NR1H4 antibody
      • NR1H4_HUMAN antibody
      • Nuclear receptor subfamily 1 group H member 4 antibody
      • Retinoid X receptor interacting protein 14 antibody
      • Retinoid X receptor-interacting protein 14 antibody
      • RIP 14 antibody
      • RIP14 antibody
      • RXR interacting protein 14 antibody
      • RXR-interacting protein 14 antibody
      see all

    Anti-Bile Acid Receptor NR1H4 antibody images

    • Anti-Bile Acid Receptor NR1H4 antibody (ab58559) at 1 µg/ml + Human Lung lysate (35ug protein in RIPA buffer).

      Predicted band size : 56 kDa
      Observed band size : 56 kDa

    References for Anti-Bile Acid Receptor NR1H4 antibody (ab58559)

    ab58559 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 50 µg
    Gel Running Conditions Reduced Denaturing (7.5%)
    Sample Rabbit Tissue lysate - whole (liver)
    Specification liver
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jun 25 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"