Anti-Bile salt-activated lipase antibody (ab87431)

Overview

  • Product nameAnti-Bile salt-activated lipase antibody
    See all Bile salt-activated lipase primary antibodies
  • Description
    Rabbit polyclonal to Bile salt-activated lipase
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Drosophila melanogaster, Non Human Primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Bile salt-activated lipase.

    (Peptide available as ab87432.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Pancreas; Mouse Pancreas

Properties

Applications

Our Abpromise guarantee covers the use of ab87431 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 78 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • RelevanceDefects in Bile salt activated lipase (CEL) are a cause of maturity onset diabetes of the young type 8 with exocrine dysfunction (MODY8) [MIM:609812], also known as diabetes and pancreatic exocrine dysfunction (DPED). MODY [MIM:606391] is an autosomal dominant form of diabetes mellitus. The pancreas serves both endocrine and exocrine functions. The endocrine cells are found in the islets of Langerhans. They synthesize insulin and other hormones, and are involved in the pathogenesis of diabetes mellitus. The exocrine cells produce bicarbonate and digestive enzymes and are involved in the pathogenesis of pancreatic malabsorption. The localization of the islets within exocrine pancreatic tissue is suggestive of an interdependency and cross talk between these two cell populations in their normal and in their abnormal function.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • BAL antibody
    • Bile salt-stimulated lipase antibody
    • BSDL antibody
    • BSSL antibody
    • Bucelipase antibody
    • Carboxyl ester lipase (bile salt stimulated lipase) antibody
    • Carboxyl ester lipase antibody
    • CEase antibody
    • CEL antibody
    • CELL antibody
    • Cholesterol esterase antibody
    • FAP antibody
    • FAPP antibody
    • Fetoacinar pancreatic protein antibody
    • LIPA antibody
    • Lysophospholipase, pancreatic antibody
    • MODY8 antibody
    • Pancreatic lysophospholipase antibody
    • Sterol esterase antibody
    see all

Anti-Bile salt-activated lipase antibody images

  • All lanes : Anti-Bile salt-activated lipase antibody (ab87431) at 1 µg/ml

    Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 2 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa (possible cleavage fragment).

    Exposure time : 30 seconds
  • All lanes : Anti-Bile salt-activated lipase antibody (ab87431) at 1 µg/ml

    Lane 1 : Human pancreas tissue lysate - total protein (ab29816)
    Lane 2 : Pancreas (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 78 kDa
    Observed band size : 74,100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 72 kDa (possible cleavage fragment).

    Exposure time : 30 seconds
  • IHC image of Bile salt-activated lipase staining in mouse pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab87431, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-Bile salt-activated lipase antibody (ab87431)

ab87431 has not yet been referenced specifically in any publications.

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