• Product nameAnti-Bim antibody
    See all Bim primary antibodies
  • Description
    Rabbit polyclonal to Bim
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Peptide corresponding to amino acids 22 to 40 of human origin. The sequence is identical to that of mouse and differs from that of rat by one amino acid.

  • Positive control
    • Human K562 cell lysate

Associated products


Our Abpromise guarantee covers the use of ab7888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 µg/ml.
WB 1/1000. Predicted molecular weight: 23 kDa.Can be blocked with Bim peptide (ab7887).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionInduces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • Tissue specificityIsoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • Sequence similaritiesBelongs to the Bcl-2 family.
  • DomainThe BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • Cellular localizationMitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all

Anti-Bim antibody images

  • All lanes : Anti-Bim antibody (ab7888) at 1 µg/ml

    Lane 1 : K562 whole cell lysate
    Lane 2 : A549 whole cell lysate

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
  • ab7888 at 20µg/ml staining Bim in human cancer cells by IHC-P

  • ICC/IF image of ab7888 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7888, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Bim antibody (ab7888)

This product has been referenced in:
  • Liu L  et al. SIRT2 enhances 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced nigrostriatal damage via apoptotic pathway. Front Aging Neurosci 6:184 (2014). WB ; Mouse . Read more (PubMed: 25157229) »
  • Liu L  et al. Sirtuin 2 (SIRT2) Enhances 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Nigrostriatal Damage via Deacetylating Forkhead Box O3a (Foxo3a) and Activating Bim Protein. J Biol Chem 287:32307-11 (2012). WB . Read more (PubMed: 22898818) »

See all 3 Publications for this product

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I am inclined to believe that the issue is with the samples themselves. It is very unusual for 3 different antibodies to detect bands mainly above 100kDa when the expected MW should be around 25kDa. How long ar...

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I was wondering if you would clarify a few additional points for me.

How were the samples prepared? How much is loaded on the gel?

What is the molecular weight of each band in the ladd...

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