Anti-Bim antibody (ab7888)
Key features and details
- Rabbit polyclonal to Bim
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Bim antibody
See all Bim primary antibodies -
Description
Rabbit polyclonal to Bim -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Peptide corresponding to amino acids 22 to 40 of human origin. The sequence is identical to that of mouse and differs from that of rat by one amino acid.
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Positive control
- WB: Human colon and thymus tissue lysates, Rat Myeloma Cell lysate ICC/IF: K562 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 23 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis. -
Tissue specificity
Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney. -
Sequence similarities
Belongs to the Bcl-2 family. -
Domain
The BH3 motif is required for Bcl-2 binding and cytotoxicity. -
Cellular localization
Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes. - Information by UniProt
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Database links
- Entrez Gene: 10018 Human
- Entrez Gene: 64547 Rat
- Omim: 603827 Human
- SwissProt: O43521 Human
- SwissProt: O88498 Rat
- Unigene: 469658 Human
- Unigene: 82709 Rat
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Alternative names
- BCL2 like 11 antibody
- B2L11_HUMAN antibody
- BAM antibody
see all
Images
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All lanes : Anti-Bim antibody (ab7888) at 0.5 µg/ml
Lane 1 : Human thymus tissue lysate
Lane 2 : Human colon tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 23 kDa -
ab7888 at 20µg/ml staining Bim in human cancer cells by IHC-P
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Anti-Bim antibody (ab7888) at 0.5 µg/ml + Rat Myeloma Cell lysate at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 23 kDa -
Immunocytochemistry/ Immunofluorescence analysis of 4% paraformaldehyde fixed human K562 cells labeling Bim with ab7888 at 20 μg/mL. Goat anti-rabbit IgG secondary antibody at 1/500 dilution was used as the secondry antibody.
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ICC/IF image of ab7888 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7888, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab7888 has been referenced in 22 publications.
- Song J et al. The 14-3-3s protein promotes HCC anoikis resistance by inhibiting EGFR degradation and thereby activating the EGFR-dependent ERK1/2 signaling pathway. Theranostics 11:996-1015 (2021). PubMed: 33391517
- Zhang D et al. CPNE3 regulates the cell proliferation and apoptosis in human Glioblastoma via the activation of PI3K/AKT signaling pathway. J Cancer 12:7277-7286 (2021). PubMed: 35003348
- Liu XK et al. Regulatory Effect of Sishen Pill on Tfh Cells in Mice With Experimental Colitis. Front Physiol 11:589 (2020). PubMed: 32581849
- Zhang J et al. MicroRNA-132 protects H9c2 cells against oxygen and glucose deprivation-evoked injury by targeting FOXO3A. J Cell Physiol 235:176-184 (2020). PubMed: 31210352
- Mei X et al. lncRNA-NR024118 overexpression reverses LPS-induced inflammatory injury and apoptosis via NF-?B/Nrf2 signaling in ATDC5 chondrocytes. Mol Med Rep 20:3867-3873 (2019). PubMed: 31485657