Validated using a knockout cell line
Recombinant
RabMAb

Anti-Bim antibody [Y36] - BSA and Azide free (ab170589)

Overview

  • Product name
    Anti-Bim antibody [Y36] - BSA and Azide free
    See all Bim primary antibodies
  • Description
    Rabbit monoclonal [Y36] to Bim - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, IHC-P, ICC, WBmore details
  • Species reactivity
    Reacts with: Human
  • General notes

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab170589 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Please check the parent abID, ab32158, for a recommended dilution.

Target

  • Function
    Induces apoptosis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than the isoforms BimEL, BimL and BimS. Isoform Bim-gamma induces apoptosis.
  • Tissue specificity
    Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are ubiquitously expressed with a tissue-specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    The BH3 motif is required for Bcl-2 binding and cytotoxicity.
  • Cellular localization
    Mitochondrion and Endomembrane system. Associated with intracytoplasmic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCL2 like 11 antibody
    • B2L11_HUMAN antibody
    • BAM antibody
    • Bcl 2 interacting protein Bim antibody
    • Bcl 2 related ovarian death agonist antibody
    • Bcl-2-like protein 11 antibody
    • BCL2 interacting mediator of cell death antibody
    • BCL2 like 11 (apoptosis facilitator) antibody
    • BCL2 like protein 11 antibody
    • Bcl2-interacting mediator of cell death antibody
    • Bcl2-L-11 antibody
    • Bcl2l11 antibody
    • BIM alpha6 antibody
    • BIM antibody
    • BIM beta6 antibody
    • BIM beta7 antibody
    • BimEL antibody
    • BimL antibody
    • BOD antibody
    see all

Images

  • Anti-Bim antibody [Y36] - BSA and Azide free (ab170589) + A431 (human epidermoid carcinoma) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 22 kDa


    Exposure time: 3 minutes


    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

References

ab170589 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab170589.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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