BIN1 antibody [99D] (ab10532)

Product Name 

BIN1 antibody [99D]

Product type 

Primary antibodies

Description 

Mouse monoclonal [99D] to BIN1

Immunogen 

Recombinant fragment, corresponding to amino acids 189-398 of Human BIN1.

Reacts with 
(species key)

Hu, Ms, Rat, Bird, Rb

Specificity 

Does not react with the Bin1 related 45 kD polypeptide.

Epitope 

Reacts specifically with a 33-residue segment of the myc binding domain (MBD, amino acids 323-356) of the human Bin1 molecule.

Tested applications 
(see key)

ELISA, Flow Cyt, ICC, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/50000. Detects a band of approximately 60-75 kDa (predicted molecular weight: 77 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Cytoplasmic (Isoform IIa) and Nuclear (isoform BIN1)

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

Relevance 

The identification and analysis of tumor suppressors is of major importance for improved diagnosis and treatment of tumors. Bin1 (Box-dependent myc interacting protein 1, also known as SH3P9, amphiphysin II and amphl/amphiphysin-like) is a novel protein that has features of a tumor suppressor. Bin1 interacts with and inhibits the oncogenic activity of the myc oncoprotein that has a major role in many human cancers. The loss of Bin1 may contribute to growth deregulation in cancer cells in carcinoma of the breast, colon, lung, cervix, prostate and liver. Bin1 also interacts with HCV NS5A through its SH3 domain.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

99D

Isotype 

IgG2b

Purity 

Ascites

Storage buffer 

Preservative: 15mM Sodium Azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.700 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for BIN1 antibody [99D] (ab10532)

  This product has been used in: (two most recent references)

English JA et al. 2-D DIGE analysis implicates cytoskeletal abnormalities in psychiatric disease. Proteomics 9:3368-82 (2009). WB, ELISA; Human. PubMed: 19562803

Capurso G et al. Gene expression profiles of progressive pancreatic endocrine tumours and their liver metastases reveal potential novel markers and therapeutic targets. Endocr Relat Cancer 13:541-58 (2006). IHC-P; Human. PubMed: 16728581
If you publish research using ab10532 please let us know so that we can cite the reference on this datasheet.

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