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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-BIN1 antibody
See all BIN1 products (9) ...
Description
Rabbit polyclonal to BIN1
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide (Mouse) - corresponding to region surrounding amino acid 578 of mouse BIN 1.
Positive control
Jurkat cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1% BSA, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Western blot - BIN1 antibody (ab27796)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab27796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 65 kDa and a 45kDa fragment can be detected in apoptotic samples.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
Tissue specificity
Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
Involvement in disease
Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
Sequence similarities
Contains 1 BAR domain.
Contains 1 SH3 domain.
Post-translational
modifications
Phosphorylated by protein kinase C.
Cellular localization
Cytoplasm and Nucleus.
Target information above from: UniProt accessionO00499
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Amphiphysin like protein antibody
- AMPH 2 antibody
- AMPH2 antibody
see all
Database links
- Entrez Gene: 274 Human
- Entrez Gene: 30948 Mouse
- Entrez Gene: 117028 Rat
- Omim: 601248 Human
- SwissProt: O00499 Human
- SwissProt: O08539 Mouse
- SwissProt: O08839 Rat
- Unigene: 193163 Human
see all
Anti-BIN1 antibody images:
Western blot - BIN1 antibody (ab27796)
All lanes : Anti-BIN1 antibody (ab27796) at 2 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 2 : Jurkat cells treated with camptothecin for 6 hours
Predicted band size : 65 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa (possible cleavage fragment).
References for Anti-BIN1 antibody (ab27796)
ab27796 has not yet been referenced specifically in any publications.
Publishing research using ab27796? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"