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Anti-BIN1 antibody (ab27796)

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Overview

Product name

Anti-BIN1 antibody
See all BIN1 products (9) ...

Description

Rabbit polyclonal to BIN1

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide (Mouse) - corresponding to region surrounding amino acid 578 of mouse BIN 1.

Positive control

Jurkat cells

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1% BSA, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - BIN1 antibody (ab27796)Western blot - BIN1 antibody (ab27796) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab27796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 65 kDa and a 45kDa fragment can be detected in apoptotic samples.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.

Tissue specificity

Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.

Involvement in disease

Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.

Sequence similarities

Contains 1 BAR domain.
Contains 1 SH3 domain.

Post-translational
modifications

Phosphorylated by protein kinase C.

Cellular localization

Cytoplasm and Nucleus.

Target information above from: UniProt accessionO00499 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Amphiphysin like protein antibody
  • AMPH 2 antibody
  • AMPH2 antibody
  • AMPH2 antibody
  • Amphiphysin 2 antibody
  • Amphiphysin II antibody
  • Amphiphysin like protein antibody
  • amphiphysin-like antibody
  • Amphiphysin-like protein antibody
  • AMPHL antibody
  • Bin1 antibody
  • BIN1_HUMAN antibody
  • Box Dependant MYC Interacting Protein 1 antibody
  • box dependant MYC interacting protein 1 antibody
  • Box-dependent myc-interacting protein 1 antibody
  • Bridging Integrator 1 antibody
  • Bridging Integrator 1 antibody
  • DKFZp547F068 antibody
  • MGC10367 antibody
  • MGC105358 antibody
  • Myc box dependent interacting protein 1 antibody
  • Myc box-dependent-interacting protein 1 antibody
  • SH3P9 antibody
  • SH3P9 antibody
see all

Anti-BIN1 antibody images:

  Western blot - BIN1 antibody (ab27796)

Western blot - BIN1 antibody (ab27796)

All lanes : Anti-BIN1 antibody (ab27796) at 2 µg/ml

Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 2 : Jurkat cells treated with camptothecin for 6 hours


Predicted band size : 65 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa (possible cleavage fragment).

References for Anti-BIN1 antibody (ab27796)

ab27796 has not yet been referenced specifically in any publications.

Publishing research using ab27796? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"