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Western blot - BIN1 antibody (ab27796)
Western blot - BIN1 antibody (ab27796)
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Product Name 

BIN1 antibody

 

See all BIN1 antibodies (5)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to BIN1

Immunogen 

Synthetic peptide (Mouse) - corresponding to region surrounding amino acid 578 of mouse BIN 1.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 0.5 - 2.0 µg/ml. Predicted molecular weight: 65 kDa and a 45kDa fragment can be detected in apoptotic samples.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Jurkat cells

Cellular localization 

Cytoplasmic (Isoform IIa) and Nuclear (isoform BIN1)

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Cancer >> Cell cycle >> Cell cycle inhibitors >> Other
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Other
Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Tumor Suppressors
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

Relevance 

The identification and analysis of tumor suppressors is of major importance for improved diagnosis and treatment of tumors. Bin1 (Box-dependent myc interacting protein 1, also known as SH3P9, amphiphysin II and amphl/amphiphysin-like) is a novel protein that has features of a tumor suppressor. Bin1 interacts with and inhibits the oncogenic activity of the myc oncoprotein that has a major role in many human cancers. The loss of Bin1 may contribute to growth deregulation in cancer cells in carcinoma of the breast, colon, lung, cervix, prostate and liver. Bin1 also interacts with HCV NS5A through its SH3 domain.

 

BIN1 antibody (ab27796)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

274    (Human)
30948    (Mouse)
117028    (Rat)

GeneCard

    

GC02M127710    (Human)

Omim

    

601248    (Human)

SwissProt

    

O00499    (Human)
O08539    (Mouse)
O08839    (Rat)

Unigene

    

193163    (Human)
44463    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1% BSA, PBS

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Thimerosal MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this BIN1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for BIN1 antibody images, references, products related to ab27796 and other tools.

 

BIN1 antibody images:

Product image  Western blot - BIN1 antibody (ab27796)

Western blot - BIN1 antibody (ab27796)

All lanes : BIN1 antibody (ab27796) at 2 µg/ml

Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lane 2 : Jurkat cells treated with camptothecin for 6 hours


Predicted band size : 65 kDa
Observed band size : 64 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa (possible cleavage fragment).

 
 

Search PubMed (MEDLINE) for references to BIN1

 

BIN1 antibody - more information

Customer reviews (feedback) regarding BIN1 antibody

Customer FAQs regarding BIN1 antibody

Protocols for BIN1 antibody

Price and availability of products related to BIN1 antibody

 

BIN1 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for BIN1 antibody

ab7899

  

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

 

Compatible Secondaries for BIN1 antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other BIN1 Antibodies

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BIN1 antibody - other tools:

Contact Abcam with a Technical Enquiry about BIN1 antibody

 

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