Overview

  • Product nameAnti-Biotin antibody [39-15D9]
    See all Biotin primary antibodies
  • Description
    Mouse monoclonal [39-15D9] to Biotin
  • SpecificityThis antibody reacts with both free biotin and biotin bound to a carrier protein. There is no cross reaction with the free carrier protein (determined by ELISA).
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Immunogen

    Full length protein conjugated to KLH.

Properties

Applications

Our Abpromise guarantee covers the use of ab3780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000 - 1/10000.
WB 1/1000 - 1/5000.

Target

  • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

References for Anti-Biotin antibody [39-15D9] (ab3780)

This product has been referenced in:
  • Potluri LP  et al. ZipA is required for FtsZ-dependent preseptal peptidoglycan synthesis prior to invagination during cell division. J Bacteriol 194:5334-42 (2012). Read more (PubMed: 22843850) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. This antibody does bind the d-isomer of biotin. Please do not hesitate to contact us if you have additional questions.

Thank you for your enquiry. The hybridoma cell line comes from the antibody's originator (the source's name is proprietary) and unfortunately we do not have any references at this time. If you have any additional questions, please contact us again.

Thank you for your enquiry. We are sorry to inform you that there is no concentration of the immunoglobulin for antibodies that are not affinity purified. Please take a look at the suggested dilutions for each application listed on the datasheet.

Read More

On Western blot membran you can detect biotinylated proteins but not free biotine. Therefore the size of the bands depends of the molecular weight of the biotintylated protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"