Anti-Biotin antibody [3E6] (ab36406)

Overview

  • Product nameAnti-Biotin antibody [3E6]
    See all Biotin primary antibodies
  • Description
    Mouse monoclonal [3E6] to Biotin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Immunogen

    Chemical/ Small Molecule - biotinylated denatured Ovalbumin.

  • General notes


    Anti biotin antibodies can be used to detect both biotinylated proteins and nucleic acids.

Properties

Applications

Our Abpromise guarantee covers the use of ab36406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. In WB this antibody reacts with biotinylated proteins in both reduced and non-reduced forms.
ELISA 1/20000. Strong reaction is seen in ELISA with biotin/ovalbumin coated onto the microtiter well and also in sandwich ELISA in combination with streptavidin.

Target

  • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

References for Anti-Biotin antibody [3E6] (ab36406)

ab36406 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your inquiry and your interest in our products.

I will have to double-check with the lab regarding glycosylation of the antibodies since I do not have this information readily at hand. I will let you know once I have received th...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (In Vitro Translation)
Loading amount 0.005 µg
Specification In Vitro Translation
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"