Overview

  • Product name
  • Description
    Rabbit polyclonal to Biotin
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WB, ELISA, IPmore details
  • Immunogen

    Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.

Properties

Applications

Our Abpromise guarantee covers the use of ab1227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ICC/IF 1/200 - 1/2000.
WB 1/1000 - 1/30000.
ELISA 1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.
IP Use at an assay dependent concentration.

Target

  • Relevance
    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

Images

  • Anti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.

    Secondary
    Streptavidin-HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

    This image is courtesy of an Abreview submitted by Mr John Andersen

    See Abreview

References

This product has been referenced in:
  • Banuelos CA  et al. Sintokamide A Is a Novel Antagonist of Androgen Receptor That Uniquely Binds Activation Function-1 in Its Amino-terminal Domain. J Biol Chem 291:22231-22243 (2016). Read more (PubMed: 27576691) »
  • Waaijers S  et al. A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large. BMC Biol 14:66 (2016). WB . Read more (PubMed: 27506200) »

See all 5 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293 cells)
Specification
293 cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - nuclear (mouse liver)
Total protein in input
50 µg
Specification
mouse liver
Immuno-precipitation step
Protein A/G
Username

Abcam user community

Verified customer

Submitted Jan 30 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse ES cells)
Specification
mouse ES cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 30 2012

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse liver)
Loading amount
50 µg
Specification
mouse liver
Gel Running Conditions
Reduced Denaturing (8% Precast Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jan 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HEK293)
Total protein in input
2000 µg
Specification
HEK293
Immuno-precipitation step
Other - Protein G Dynabeads
Username

Abcam user community

Verified customer

Submitted Dec 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - nuclear (HEK293-T)
Loading amount
100 µg
Specification
HEK293-T
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Dec 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (Ovarian tissue)
Loading amount
50 µg
Specification
Ovarian tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. John Andersen

Verified customer

Submitted Sep 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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