Overview

  • Product nameAnti-Biotin antibody
    See all Biotin primary antibodies
  • Description
    Rabbit polyclonal to Biotin
  • Tested applicationsSuitable for: WB, ELISA, IPmore details
  • Immunogen

    Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.

Properties

Applications

Our Abpromise guarantee covers the use of ab1227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/30000. Predicted molecular weight: 80 kDa.
ELISA 1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.
IP Use at an assay dependent concentration.

Target

  • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

Anti-Biotin antibody images

  • Anti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.

    Secondary
    Streptavidin-HRP
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 80 kDa


    Exposure time : 10 seconds

    This image is courtesy of an Abreview submitted by Mr John Andersen

    See Abreview

References for Anti-Biotin antibody (ab1227)

This product has been referenced in:
  • Brown XQ  et al. Effect of substrate stiffness and PDGF on the behavior of vascular smooth muscle cells: implications for atherosclerosis. J Cell Physiol 225:115-22 (2010). ELISA . Read more (PubMed: 20648629) »
  • Morais S  et al. Multiplexed microimmunoassays on a digital versatile disk. Anal Chem 81:5646-54 (2009). ELISA . Read more (PubMed: 19522512) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (293 cells)
Specification 293 cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Submitted Jan 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - nuclear (mouse liver)
Total protein in input 50 µg
Specification mouse liver
Immuno-precipitation step Protein A/G
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Submitted Jan 30 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse ES cells)
Specification mouse ES cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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Verified customer

Submitted Jan 30 2012

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mouse liver)
Loading amount 50 µg
Specification mouse liver
Gel Running Conditions Reduced Denaturing (8% Precast Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jan 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - nuclear (HEK293)
Total protein in input 2000 µg
Specification HEK293
Immuno-precipitation step Other - Protein G Dynabeads
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Submitted Dec 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HEK293-T)
Loading amount 100 µg
Specification HEK293-T
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Dec 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Ovarian tissue)
Loading amount 50 µg
Specification Ovarian tissue
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Mr. John Andersen

Verified customer

Submitted Sep 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"