Overview

  • Product nameAnti-Biotin antibody
    See all Biotin primary antibodies
  • Description
    Rabbit polyclonal to Biotin
  • SpecificityReacts with free biotin and biotinylated antibody and proteins
  • Tested applicationsSuitable for: WB, Competitive ELISA, IP, ICC/IFmore details
  • Immunogen

    Full length protein conjugated to KLH

Properties

Applications

Our Abpromise guarantee covers the use of ab53494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.
Competitive ELISA Use at an assay dependent dilution. (sensitivity: 0.01 ng/mL).
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.

Target

  • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

References for Anti-Biotin antibody (ab53494)

This product has been referenced in:
  • Caron JM & Caron JM Methyl Sulfone Blocked Multiple Hypoxia- and Non-Hypoxia-Induced Metastatic Targets in Breast Cancer Cells and Melanoma Cells. PLoS One 10:e0141565 (2015). Read more (PubMed: 26536104) »
  • Eichelbaum K & Krijgsveld J Rapid temporal dynamics of transcription, protein synthesis, and secretion during macrophage activation. Mol Cell Proteomics 13:792-810 (2014). WB . Read more (PubMed: 24396086) »

See all 3 Publications for this product

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (RWP1)
Specification RWP1
Total protein in input 500 µg
Username

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Verified customer

Submitted Feb 14 2014

Thank you for contacting us.

The optimal dilutions or concentrations has to be determined by end user. We can recommend using the antibody at starting dilution 10ug/ml.

I hope this information is helpful to you. Please do not hesitate...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"