SpecificityThe antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immunohistochemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
In situ hybridization
Application notesDot: 1/4000.
ISH: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Vitamin B4 antibody
References for Anti-Biotin antibody [BN-34] (HRP) (ab49358)
has not yet been referenced specifically in any publications.
Publishing research using ab49358? Please let us know so that we can cite the reference in this datasheet.
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