Anti-Biotin antibody [BN-34] (HRP) (ab49358)

Overview

  • Product nameAnti-Biotin antibody [BN-34] (HRP)
    See all Biotin primary antibodies
  • Description
    Mouse monoclonal [BN-34] to Biotin (HRP)
  • ConjugationHRP
  • SpecificityThe antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins
  • Tested applicationsSuitable for: In situ hybridization, IHC-P, Indirect ELISA, Dot blot, FluroSpotmore details
  • Immunogen

    Biotin conjugated to KLH

  • Positive control
    • Human tonsils and human IgG
  • General notes

    The immunoglobulin G fraction of the ascites fluid is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde.

Properties

Applications

Our Abpromise guarantee covers the use of ab49358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
In situ hybridization
IHC-P
Indirect ELISA
Dot blot
FluroSpot
  • Application notesDot: 1/4000.
    Fluorospot: 1/80000.
    IHC-P: 1/300.
    ISH: Use at an assay dependent dilution.
    I-ELISA: 1/30000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
    • Alternative names
      • Vitamin B4 antibody

    References for Anti-Biotin antibody [BN-34] (HRP) (ab49358)

    ab49358 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"