Anti-Biotin antibody (FITC) (ab53469)

Overview

  • Product nameAnti-Biotin antibody (FITC)
    See all Biotin primary antibodies
  • Description
    Rabbit polyclonal to Biotin (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityReacts with free biotin and biotinylated proteins regardless of species.
  • Tested applicationsSuitable for: ICC/IFmore details
  • Immunogen

    d-Biotin conjugated to KLH

  • General notes

    Fluorescein isothionate; Ab/FITC ratio=approximately 1/20.

Properties

Applications

Our Abpromise guarantee covers the use of ab53469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

Target

  • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

Anti-Biotin antibody (FITC) images

  • ab53469 staining Biotin in human ovarian cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1% Triton ×100 in PBS and blocking with 5% serum for 30min at 230C was performed. Samples were incubated with primary antibody (1/100) for 1 hour at 23°C.

    See Abreview

References for Anti-Biotin antibody (FITC) (ab53469)

ab53469 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (ovarian)
Specification ovarian
Fixative Formaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jun 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"