Overview

  • Product nameAnti-Biotin antibody (FITC)
    See all Biotin primary antibodies
  • Description
    Goat polyclonal to Biotin (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cyt, ICC/IF, IHC-FoFrmore details
  • Immunogen

    Chemical/ Small Molecule conjugated to KLH

  • General notes

    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG

Properties

Applications

Our Abpromise guarantee covers the use of ab6650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab37394 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/500 - 1/2500.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19255771

Target

  • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

References for Anti-Biotin antibody (FITC) (ab6650)

This product has been referenced in:
  • Baratta JL  et al. Cellular organization of normal mouse liver: a histological, quantitative immunocytochemical, and fine structural analysis. Histochem Cell Biol 131:713-26 (2009). IHC-FoFr . Read more (PubMed: 19255771) »

See 1 Publication for this product

Product Wall

The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 2-5:1

To our knowledge this antibody has yet to be tested in this application. We would welcome any feedback in this respect from researchers who have tried using this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"