HRP Anti-Biotin antibody (ab19221)
Key features and details
- HRP Goat polyclonal to Biotin
- Suitable for: ELISA, WB, ICC
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Biotin antibody
See all Biotin primary antibodies -
Description
HRP Goat polyclonal to Biotin -
Host species
Goat -
Conjugation
HRP -
Tested applications
Suitable for: ELISA, WB, ICCmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin.
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General notes
Molar enzyme/antibody protein ratio is 4:1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: PBS, 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Related Products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
1/10000 - 1/100000.
|
|
WB |
1/1000.
|
|
ICC |
1/200 - 1/500.
|
Notes |
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ELISA
1/10000 - 1/100000. |
WB
1/1000. |
ICC
1/200 - 1/500. |
Target
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Relevance
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
Alternative names
- Vitamin B4 antibody
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab19221 has been referenced in 6 publications.
- Song Z et al. Development of a CD63 Aptamer for Efficient Cancer Immunochemistry and Immunoaffinity-Based Exosome Isolation. Molecules 25:N/A (2020). PubMed: 33261145
- Becker T et al. Genetic information from discordant sibling pairs points to ESRP2 as a candidate trans-acting regulator of the CF modifier gene SCNN1B. Sci Rep 10:22447 (2020). PubMed: 33384439
- Wang T et al. Efficient Epidermal Growth Factor Receptor Targeting Oligonucleotide as a Potential Molecule for Targeted Cancer Therapy. Int J Mol Sci 20:N/A (2019). PubMed: 31546749
- Khalil S et al. Iron modulation of erythropoiesis is associated with Scribble-mediated control of the erythropoietin receptor. J Exp Med 215:661-679 (2018). PubMed: 29282252
- Wei W et al. Soluble Frizzled-7 receptor inhibits Wnt signaling and sensitizes hepatocellular carcinoma cells towards doxorubicin. Mol Cancer 10:16 (2011). WB . PubMed: 21314951
- Bühler P et al. Influence of structural variations on biological activity of anti-PSMA scFv and immunotoxins targeting prostate cancer. Anticancer Res 30:3373-9 (2010). WB . PubMed: 20944111