Overview

  • Product nameAnti-Biotin antibody (HRP)
    See all Biotin primary antibodies
  • Description
    Rabbit polyclonal to Biotin (HRP)
  • ConjugationHRP
  • SpecificityReacts with free biotin and biotinylated antibody and proteins.
  • Tested applicationsSuitable for: WB, Competitive ELISAmore details
  • Immunogen

    d-Biotin conjugated to KLH

  • General notes

    Reductive amination; Ab/HRP ratio=1/10.

Properties

Applications

Our Abpromise guarantee covers the use of ab53468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Competitive ELISA
  • Application notescELISA: Use at an assay dependent dilution; detection sensitivity of 0.01 ng/mL
    WB: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
    • Alternative names
      • Vitamin B4 antibody

    References for Anti-Biotin antibody (HRP) (ab53468)

    ab53468 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"