Anti-Biotin antibody (HRP) (ab6651)

Overview

  • Product nameAnti-Biotin antibody (HRP)
    See all Biotin primary antibodies
  • Description
    Goat polyclonal to Biotin (HRP)
  • ConjugationHRP
  • Tested applicationsSuitable for: Dot blot, ELISA, IHC-P, IHC-Fr, WBmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Biotin conjugated to Keyhole Limpet Haemocyanin (KLH).

Properties

Applications

Our Abpromise guarantee covers the use of ab6651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
ELISA 1/140000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
WB 1/3000 - 1/15000.

Target

  • RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

References for Anti-Biotin antibody (HRP) (ab6651)

ab6651 has not yet been referenced specifically in any publications.

Product Wall

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While it is theoretically possible to amplify your signal by using a biotinylated secondary antibody with an anti-biotin tertiary antibody, it is not commonly done. Because streptavidin binds to biotin with a very...

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Thank you for contacting us. I see no reason why anti-biotin antibody ab6651 or HRP conjugated-streptavedin protein ab7403 would not recognise the biotin conjugated to your customers protein of interest following a boiling step. Biotinylated protein la...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"