Overview

  • Product name
    Anti-Biotin antibody [SB58c]
    See all Biotin primary antibodies
  • Description
    Mouse monoclonal [SB58c] to Biotin
  • Tested applications
    Suitable for: Flow Cyt, Indirect ELISA, ICC/IFmore details
  • Species reactivity
    Not applicable.
  • Immunogen

    Biotin conjugated to KLH

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab79111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Indirect ELISA Use a concentration of 1 µg/ml.
ICC/IF Use 1µg for 106 cells.

Target

  • Relevance
    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • Alternative names
    • Vitamin B4 antibody

References for Anti-Biotin antibody [SB58c] (ab79111)

This product has been referenced in:
  • Workman ER  et al. Rapid antidepressants stimulate the decoupling of GABA(B) receptors from GIRK/Kir3 channels through increased protein stability of 14-3-3?. Mol Psychiatry 20:298-310 (2015). ICC/IF . Read more (PubMed: 25560757) »

See 1 Publication for this product

Product Wall

Thank you for contacting Abcam.

I am not familiar with the assay that you are using and so I am not sure if I can answer your first question, but I will speak to some of my colleagues and see if there is anything they can suggest. I will let ...

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