General notesab99714 Anti-Biotin can be used for indirect immuno-staining when working with a biotinylated primary antibody. It can be used for simultaneous staining of several biotinylated antibodies as one of the parameters in certain assay platforms (such as Luminex). It is an alternative technology to magnify the signal and to reduce background caused by avidin or its derivatives.
Storage instructionsShipped at 4°C. Store at +4°C.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
1/4000 - 1/8000.
RelevanceBiotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
Vitamin B4 antibody
Anti-Biotin antibody [SB58c] (HRP) images
ELISA - Mouse monoclonal [SB58c] Secondary Antibody to (HRP) (ab99714)
Elisa showing sensitivity of Human IL-7 detected with ab99714 6.9pg/ml and with HRP-Straptavidin 10.5pg/ml
References for Anti-Biotin antibody [SB58c] (HRP) (ab99714)
has not yet been referenced specifically in any publications.
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