Anti-Biotin Ligase Epitope Tag antibody (ab106159)

Overview

  • Product nameAnti-Biotin Ligase Epitope Tag antibody
  • Description
    Rabbit polyclonal to Biotin Ligase Epitope Tag
  • Specificityab106159 detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BirA). The antibody will recognize the BirA tag irrespective of whether the tag is biotinylated.
    Western blot analysis of Bacillus subtilis strains producing either BirA-tagged SpoIIIAH or BirA-tagged SpoIIQ showed strong and specific reactivity to the antisera.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Other
  • Immunogen

    Synthetic peptide corresponding to Escherichia coli Biotin Ligase Epitope Tag.
    Sequence:

    GLNDIFEAQKIEWH

  • Positive control
    • Whole cell lysates from Bacillus subtilis producing BirA-tagged SpoIIIAH or BirA-tagged SpoIIQ
  • General notesDilute only prior to immediate use.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.

References for Anti-Biotin Ligase Epitope Tag antibody (ab106159)

ab106159 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - nuclear (Hek293T)
Gel Running Conditions Reduced Denaturing (4–15% Mini Protean TGX (Bio-Rad))
Loading amount 30 µg
Treatment Biotin 50 uM for 24 h
Specification Hek293T
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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Submitted Oct 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"