Anti-Biotin Ligase Epitope Tag antibody (ab106159)

Overview

  • Product name
    Anti-Biotin Ligase Epitope Tag antibody
  • Description
    Rabbit polyclonal to Biotin Ligase Epitope Tag
  • Specificity
    ab106159 detects the minimal peptide substrate (GLNDIFEAQKIEWH) for the E. coli biotin ligase tag (BirA). The antibody will recognize the BirA tag irrespective of whether the tag is biotinylated.
    Western blot analysis of Bacillus subtilis strains producing either BirA-tagged SpoIIIAH or BirA-tagged SpoIIQ showed strong and specific reactivity to the antisera.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Other
  • Immunogen

    Synthetic peptide corresponding to Escherichia coli Biotin Ligase Epitope Tag.
    Sequence:

    GLNDIFEAQKIEWH

  • Positive control
    • Whole cell lysates from Bacillus subtilis producing BirA-tagged SpoIIIAH or BirA-tagged SpoIIQ
  • General notes
    Dilute only prior to immediate use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride, Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab106159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000.

References

ab106159 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - nuclear (Hek293T)
Gel Running Conditions
Reduced Denaturing (4–15% Mini Protean TGX (Bio-Rad))
Loading amount
30 µg
Treatment
Biotin 50 uM for 24 h
Specification
Hek293T
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

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Submitted Oct 30 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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