Overview

  • Product name
    Biotin (Type A) Fast Conjugation Kit
  • Product overview

    Biotinylation Kit / Biotin Conjugation Kit (ab201795) uses an easy-to-use, one step procedure for the direct labeling of proteins, peptides, and other biomolecules containing primary amines, with biotin with only 30 seconds hands-on time; conjugates are ready to use in less than twenty minutes.


    This Biotinylation Kit / Biotin Conjugation Kit has been optimized to produce conjugates for assays in which a streptavidin-labeled detection reagent will be used.


    Use Biotinylation Kit ab201796 for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (e.g., plates, nitrocellulose, magnetic beads etc).


    Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.

  • Notes

    The kit comes in 3 sizes for labelling recommended amounts of antibody:

    30 μg containing 3 reactions (each for 10 - 20 μg)

    300 μg containing 3 reactions (each for 100 - 200 μg)

    1 mg containing 1 reaction (for 1 - 2 mg)

     

    The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab201795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

  • This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

Protocols

References

ab201795 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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