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The Abcam Biotin (Type B) Fast Conjugation Kit (ab201796) provides an easy-to-use, one step procedure that allows researchers to directly label proteins, peptides and other biomolecules containing primary amines with biotin with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.
The Abcam Biotin (Type B) Fast Conjugation Kit (ab201796) has been optimized to produce conjugates for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (e.g., plates, nitrocellulose, magnetic beads etc).
Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.
The kit comes in 3 sizes for labelling recommended amounts of antibody:
30 μg containing 3 reactions (each for 10 - 20 μg)
300 μg containing 3 reactions (each for 100 - 200 μg)
1 mg containing 1 reaction (for 1 - 2 mg)
The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.
|Components||30 µg||300 µg||1 mg|
|Biotin (Type B) Conjugation Mix||3 vials||3 vials||1 vial|
|Biotin (Type B) Modifier Reagent||1 vial||1 vial||1 vial|
|Biotin (Type B) Quencher Reagent||1 vial||1 vial||1 vial|
Our Abpromise guarantee covers the use of ab201796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Conjugation||Use at an assay dependent concentration.|
ab201796 has not yet been referenced specifically in any publications.