Overview

  • Product name
    Biotin (Type B) Fast Conjugation Kit
    See all Biotin (type B) kits
  • Product overview

    Biotinylation Kit / Biotin Conjugation Kit ab201796 provides an easy-to-use, one step procedure that allows researchers to directly label proteins, peptides and other biomolecules containing primary amines with biotin with only 30 seconds hands-on time; furthermore conjugates are ready to use in less than twenty minutes.


    This Biotinylation Kit is optimized to produce conjugates for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (e.g., plates, nitrocellulose, magnetic beads etc).


    Use Biotinylation Kit ab201795 to produce conjugates for assays in which a streptavidin-labeled detection reagent will be used.


    Learn more about buffer compatibility, protein/secondary antibody conjugation and labeling chemistry in our FAQs.

  • Notes

    The kit comes in 3 sizes for labelling recommended amounts of antibody:

    30 μg containing 3 reactions (each for 10 - 20 μg)

    300 μg containing 3 reactions (each for 100 - 200 μg)

    1 mg containing 1 reaction (for 1 - 2 mg)

     

    The antibody to be labelled should be purified, in an appropriate buffer for conjugation and at a suitable concentration, as described in the protocol booklet. If not, consider using our antibody purification and concentration kits.

  • Tested applications
    Suitable for: Conjugationmore details

Properties

Applications

Our Abpromise guarantee covers the use of ab201796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent concentration.

Images

  • This illustration demonstrates a general procedure and will slightly vary dependent on the conjugate used.

Protocols

References

This product has been referenced in:
  • Lino Cardenas CL  et al. An HDAC9-MALAT1-BRG1 complex mediates smooth muscle dysfunction in thoracic aortic aneurysm. Nat Commun 9:1009 (2018). Read more (PubMed: 29520069) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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