Biotinylated Goat anti-Mouse IgG (H+L) (Ready to Use) (ab64255)


  • Product name
    Biotinylated Goat anti-Mouse IgG (H+L) (Ready to Use)
    See all IgG reagents
  • Tested applications
    Suitable for: Sandwich ELISA, IHC-Pmore details
  • General notes
    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
    2. Add enough drops of an approriate Hydrogen Peroxide Block to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
    3. Perform appropriate pretreatment if required. Wash 3 times in buffer.
    4. Apply an appropriate Protein Block and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash 1 time in buffer.
    5. Apply primary antibody and incubate according to manufacturer's protocol.
    6. Wash 4 times in buffer. Apply Biotinylated Goat anti Mouse IgG (H+L) solution and incubate for 10 minutes at room temperature. Wash 4 times in buffer.
    7. Apply enzyme labeled streptavidin and incubate according to manufacturer’s protocol.
    8. Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
    9. Add enough drops of Hematoxylin to cover the section. Incubate for 1 minute.
    10. Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.

    This reagent is intended to be used in a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.


  • Relevance
    IgG is the prominent type of immunoglobulin existing in the blood. There are four different subcategories of IgG, the major form of immunoglobulin. It coats germs and makes them easier for germ-eating cells to find. It is composed of a heavy chain (with Fc portion and a variable head) and a variable light chain.


Our Abpromise guarantee covers the use of ab64255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration. PubMed: 23754944
IHC-P Use at an assay dependent dilution.

Biotinylated Goat anti-Mouse IgG (H+L) (Ready to Use) images

  • Human esophagus tissue section stained with biotinylated Goat anti Mouse IgG (H+L) (ab64255).

References for Biotinylated Goat anti-Mouse IgG (H+L) (Ready to Use) (ab64255)

This product has been referenced in:
  • Gopinath S  et al. The systemic immune state of super-shedder mice is characterized by a unique neutrophil-dependent blunting of TH1 responses. PLoS Pathog 9:e1003408 (2013). Sandwich ELISA ; Mouse . Read more (PubMed: 23754944) »

See 1 Publication for this product

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The ratio is approximately 6-8 biotins per antibody.

I hope this is helpful. Please contact us again if you have any further questions.

The concentration is always 7ug/mL for this product.
Please let me know if there is additional information I can provide.

Thank you for your enquiry.

Yes you can use ab64255 for ELISA. Please note it comes pre-diluted and is meant to be used neat. Further dilution is possible but if signal intensity is low you could not increase concentration of the secondary. <...

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This is a ready to use product. You do not need to dilute it further.

However if you get high intensity of staining then you may dilute it 1/10, this way you can do more tests and antibody will last lon...

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Thank you for contacting us.

We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT ...

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Vielen Dank für Ihren Anruf. ab64255 wurde über Affinitätschromatographie aus dem Antiserum aufgereinigt; der exakte Reinheitsgrade wurde leider nicht bestimmt. Der Puffer, in dem der Antikörper vorliegt, enthält BSA. Die Konzentration beträgt 7 µg/...

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