Biotinylated Goat Anti-Rabbit IgG (H+L) (Ready to use) (ab64256)

Overview

  • Product nameBiotinylated Goat Anti-Rabbit IgG (H+L) (Ready to use)
    See all IgG reagents
  • Tested applicationsSuitable for: IHC-Pmore details
  • General notesStaining protocol
    1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
    2. Add appropriate hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer.
    3. Perform appropriate pretreatment if required. Wash 3 times in buffer.
    4. Apply appropriate protein blocking solution and incubate for 5 minutes at room temperature to block non specific background staining. Wash 1 time in buffer.
    5. Apply primary antibody and incubate according to manufacturer's protocol.
    6. Wash 4 times in buffer. Apply Ready to use Biotinylated Goat anti Rabbit IgG (ab64256) solution and incubate for 10-15 minutes at room temperature. Wash 4 times in buffer.
    7. Apply enzyme labelled streptavidin and incubate according to manufacturer’s protocol.
    8. Rinse 4 times in buffer. Place slide in appropriate chromogenic substrate and incubate until desired reaction is achieved. Rinse 4 times in buffer.
    9. Add enough drops of Haematoxylin to cover the section. Incubate for 1 minute.
    10. Rinse 7-8 times in tap water. Add Mounting Medium to cover the section.


    This reagent is intended to be used in a labeled streptavidin biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme labeled streptavidin, and substrate chromogen.

Properties

  • RelevanceIgG is the prominent type of immunoglobulin existing in the blood. There are four different subcategories of IgG, the major form of immunoglobulin. It coats germs and makes them easier for germ-eating cells to find. It is composed of a heavy chain (with Fc portion and a variable head) and a variable light chain.

Applications

Our Abpromise guarantee covers the use of ab64256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.

Biotinylated Goat Anti-Rabbit IgG (H+L) (Ready to use) images

  • Human tonsil stained with anti-CD3 antibody using Biotinylated Goat Anti Rabbit IgG (H+L) (Ready to use) (ab64256).
  • Ab64256 was used with the primary antibody ab3517 in IHC-P. See Abreview on ab3517.

References for Biotinylated Goat Anti-Rabbit IgG (H+L) (Ready to use) (ab64256)

This product has been referenced in:
  • Yao X  et al. Mitochondrial ROS Induces Cardiac Inflammation via a Pathway through mtDNA Damage in a Pneumonia-Related Sepsis Model. PLoS One 10:e0139416 (2015). IHC ; Rat . Read more (PubMed: 26448624) »
  • Ciccia F  et al. Evidence that autophagy, but not the unfolded protein response, regulates the expression of IL-23 in the gut of patients with ankylosing spondylitis and subclinical gut inflammation. Ann Rheum Dis N/A:N/A (2013). Read more (PubMed: 23740229) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us about the Biotinylated Goat Anti Rabbit IgG antibody, ab64256.

The ratio of biotin to antibody is estimated to be 6 - 8. The concentration of the antibody is 5ug/ml.

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Thank you for contacting us.

We do not currently offer double staining IHC kits. Your staining conditions are challenging as, of the two common HRP chromogens, only DAB is alcohol insoluable. While of the common AP chromagens, only BCIP/NBT ...

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Thank you for your inquiry. ab64257 is indeed suitable for the detection of Mouse and Rabbit IgG. It can be used as a secondary antibody when used with rabbit or mouse primary antibodies. About ab64256 and ab97044, all of the information we have...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"