General notesTissue: Urinary bladder
Location: Left and right walls of bladder.
Diagnosis: Adenocarcinoma. Poorly differentiated.
Age: 74 years
Gross findings: Invasive multiple tumors, sizes 28 to 55 mm.
Cut section firm, gray-white. Hemorrhagic.
Cell distribution: (Positive)
Structure / Pattern: (Positive)
Nuclear Appearance: Grades 0 I II and III
Anisonucleosis: Grade II
Hyperchromatism: Grade III
Nucleolar prominent: Grade III
Multinucleated giant cell: Grade III
Mitotic activity: Grade III
Nuclear grade: Grade III
Soluble protein fraction.
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Purification notesTissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
Lysate notesThis product belongs to a range of individual sets of lysates that were
extracted from very specific tissues and are gender-, cancer subtype-,
tumor grade- and cellular morphology- specific. They allow for
consistency, reproducibility, and comparative studies from experiment
to experiment, due to the existence of specific (matched) lysates that
can be used as controls. Two extractions from each tissue were
performed, extraction 1 yielding the soluble protein fraction and
extraction 2 the insoluble protein fraction.
Please find below a range of products that relate to ab44765:
Extraction 1: soluble protein fraction
ab44765 Human Bladder Tumor Tissue lysate
ab44763 Human Bladder Normal Tissue lysate (matched)
Extraction 2: insoluble protein fraction
ab44767 Human Bladder Tumor Tissue lysate
ab46452 Human Bladder Normal Tissue lysate (matched)
References for Bladder (Human) Tissue Lysate - Adenocarcinoma (ab44765)
has not yet been referenced specifically in any publications.
Publishing research using
? Please let us know so that we can cite the reference in this datasheet