Overview

  • Product nameAnti-BLAP75 antibody
    See all BLAP75 primary antibodies
  • Description
    Rabbit polyclonal to BLAP75
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residues 275 and 300 of human BLAP75 (NP_079221.1)

  • Positive control
    • Whole cell lysate from HeLa cells. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 70 kDa).
IP Use at 1-4 µg/mg of lysate.

Target

  • FunctionEssential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. Required for BLM phosphorylation during mitosis. Within the BLM complex, required for BLM and TOP3A stability.
  • Sequence similaritiesBelongs to the RMI1 family.
  • Cellular localizationNucleus. Forms foci in response to DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • BLAP75 antibody
    • BLM associated polypeptide 75 kDa antibody
    • BLM associated protein 75 kDa antibody
    • BLM-associated protein of 75 kDa antibody
    • C9orf76 antibody
    • FAAP75 antibody
    • FLJ12888 antibody
    • Homolog of yeast RecQ mediated genome instability 1 antibody
    • RecQ mediated genome instability 1 antibody
    • RecQ-mediated genome instability protein 1 antibody
    • Rmi1 antibody
    • RMI1 homolog antibody
    • RMI1 RecQ mediated genome instability 1 homolog antibody
    • RMI1_HUMAN antibody
    • RP11-346I8 antibody
    see all

Anti-BLAP75 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BLAP75 with ab70525 at 1/200 (1µg/ml). Detection: DAB.
  • ICC/IF image of ab70525 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab70525 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-BLAP75 antibody (ab70525) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 70 kDa
    Observed band size : 74 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,170 kDa,190 kDa,238 kDa,32 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Detection of Human BLAP75 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70525 at 3 µg/mg for IP and at 1 µg/ml for subsequent WB detection.

References for Anti-BLAP75 antibody (ab70525)

This product has been referenced in:
  • Ke Y  et al. PICH and BLM limit histone association with anaphase centromeric DNA threads and promote their resolution. EMBO J 30:3309-21 (2011). WB ; Human . Read more (PubMed: 21743438) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"