Anti-BLBP antibody (ab32423)
Key features and details
- Rabbit polyclonal to BLBP
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Zebrafish, Quail
- Isotype: IgG
Overview
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Product name
Anti-BLBP antibody
See all BLBP primary antibodies -
Description
Rabbit polyclonal to BLBP -
Host species
Rabbit -
Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab279649.
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Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Zebrafish, Quail
Predicted to work with: Chicken, Human, Pig -
Immunogen
Synthetic peptide corresponding to Mouse BLBP aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab32422) -
Positive control
- ICC/IF: Primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. cells. PC12 cells. Primary mouse neurons/glia, DIV14 cells. IHC-P: Rat embryo spinal cord, Mouse embryo spinal cord, Quail developing spinal cord, Zebrafish whole body tissue sections. WB: Mouse Brain.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (7) |
1/200 - 1/1000.
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ICC/IF | (3) |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
Abcam recommends using milk as the blocking agent. |
Notes |
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IHC-P
1/200 - 1/1000. |
ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). Abcam recommends using milk as the blocking agent. |
Target
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Function
B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers. -
Tissue specificity
Expressed in brain and other neural tissues. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 396246 Chicken
- Entrez Gene: 2173 Human
- Entrez Gene: 12140 Mouse
- Entrez Gene: 80841 Rat
- Omim: 602965 Human
- SwissProt: Q05423 Chicken
- SwissProt: O15540 Human
- SwissProt: P51880 Mouse
see all -
Alternative names
- B FABP antibody
- B-FABP antibody
- BFABP antibody
see all
Images
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ab32423 staining BLBP in primary hippocampal rat neurons/glia, (obtained from Neuromics, cat. no. PC35101), DIV14. cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab32423 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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BLBP detected on rat embryo day 16 spinal cord T/S on 5 micron thick formalin-fixed paraffin wax embedded sections using Rabbit polyclonal to BLBP (ab32423; incubated at 1/200 for 16h at RT). 1% BSA (10 mins at RT) used as blocking agent. Secondary Antibody was biotin labeled anti-rabbit IgG (1/300). Immunoreactivity was visualised by standard streptAvidin-biotin-complex using peroxidase as a reporter and DAB as chromogen following citric acid (pH 6) heat mediated antigen retrieval. Expected specific staining observed with ab32423 with low signal to noise ratio.
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Anti-BLBP antibody (ab32423) at 1/250 dilution +
Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa -
BLBP detected on mouse embryo day 16 spinal cord T/S on 5 micron thick formalin-fixed paraffin wax embedded sections using Rabbit polyclonal to BLBP (ab32423; incubated at 1/300 for 16h at RT). 1% BSA (10 mins at RT) used as blocking agent. Secondary Antibody was biotin labeled anti-rabbit IgG (1/300). Immunoreactivity was visualised by standard streptAvidin-biotin-complex using peroxidase as a reporter and DAB as chromogen following citric acid (pH 6) heat mediated antigen retrieval. Expected specific staining observed with ab32423.
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Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for BLBP antibody (ab32423) on Zebrafish Tissue sections (young adult whole body T/S). Antigen retrieval step: Heat mediated Blocking step: 1% BSA for 10 mins at RT.
Primary Antibody: ab32423 incubated at 1/500 for 2 hours at RT.
Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300). -
Immunohistochemistical detection (on Formaldehyde /PFA-fixed paraffin-embedded sections) of BLBP protein using BLBP antibody (ab32423) on Quail Tissue sections (developing spinal cord). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT.
Primary Antibody: ab32423 incubated at 1/500 for 2 hours at RT.
Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (89)
ab32423 has been referenced in 89 publications.
- Su Y et al. Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development. Neuron 111:190-201.e8 (2023). PubMed: 36384142
- Heaven MR et al. Metabolic Enzyme Alterations and Astrocyte Dysfunction in a Murine Model of Alexander Disease With Severe Reactive Gliosis. Mol Cell Proteomics 21:100180 (2022). PubMed: 34808356
- Li J et al. Auts2 deletion involves in DG hypoplasia and social recognition deficit: The developmental and neural circuit mechanisms. Sci Adv 8:eabk1238 (2022). PubMed: 35235353
- Bai Q et al. Metastatic Tumor Cell-Specific FABP7 Promotes NSCLC Metastasis via Inhibiting β-Catenin Degradation. Cells 11:N/A (2022). PubMed: 35269427
- Park JS et al. Proteomic changes in broiler liver by body weight differences under chronic heat stress. Poult Sci 101:101794 (2022). PubMed: 35334443