Overview

  • Product name
    Anti-BLK antibody - N-terminal
    See all BLK primary antibodies
  • Description
    Goat polyclonal to BLK - N-terminal
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey, Common marmoset
  • Immunogen

    Synthetic peptide corresponding to Human BLK aa 22-34 (N terminal) (Cysteine residue).
    Sequence:

    QWSPLKVSAQDKD


    Database link: P51451

  • Positive control
    • Human spleen tissue; Human thymus, spleen and lymph node tissue lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab189320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Modulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.
  • Tissue specificity
    Expressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
  • Involvement in disease
    Defects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11) [MIM:613375]. MODY11 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • B lymphocyte kinase antibody
    • B lymphoid tyrosine kinase antibody
    • Blk antibody
    • BLK nonreceptor tyrosine kinase antibody
    • BLK_HUMAN antibody
    • EC 2.7.10.2 antibody
    • MGC 10442 antibody
    • MGC10442 antibody
    • MODY 11 antibody
    • MODY11 antibody
    • P55 BLK antibody
    • p55-BLK antibody
    • Tyrosine kinase B lymphocyte specific antibody
    • Tyrosine protein kinase antibody
    • Tyrosine-protein kinase BLK antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human spleen tissue labeling BLK with ab189320 at 3.75 µg/ml.

  • Anti-BLK antibody - N-terminal (ab189320) at 0.1 µg + Human thymus tissue lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 57 kDa
    Observed band size: 50 kDa (why is the actual band size different from the predicted?)

References

ab189320 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab189320.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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