Anti-BLK (phospho Y501) antibody (ab111889)

Overview

  • Product nameAnti-BLK (phospho Y501) antibody
    See all BLK primary antibodies
  • Description
    Rabbit polyclonal to BLK (phospho Y501)
  • Specificityab111889 detects endogenous levels of BLK only when phosphorylated at tyrosine 501 (Human: Tyr501; Mouse: Tyr495; Rat: Tyr495).
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human BLK around the phosphorylation site of tyrosine 501 (R-Q-YP-E-L)

  • Positive control
    • Human breast carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111889 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionModulator of beta-cells function, acting through the up-regulation of PDX1 and NKX6-1 and consequent stimulation of insulin secretion in response to glucose.
  • Tissue specificityExpressed in lymphatic organs, pancreatic islets, Leydig cells, striate ducts of salivary glands and hair follicles.
  • Involvement in diseaseDefects in BLK are a cause of maturity-onset diabetes of the young type 11 (MODY11) [MIM:613375]. MODY11 is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • B lymphocyte kinase antibody
    • B lymphoid tyrosine kinase antibody
    • Blk antibody
    • BLK nonreceptor tyrosine kinase antibody
    • BLK_HUMAN antibody
    • EC 2.7.10.2 antibody
    • MGC 10442 antibody
    • MGC10442 antibody
    • MODY 11 antibody
    • MODY11 antibody
    • P55 BLK antibody
    • p55-BLK antibody
    • Tyrosine kinase B lymphocyte specific antibody
    • Tyrosine protein kinase antibody
    • Tyrosine-protein kinase BLK antibody
    see all

Anti-BLK (phospho Y501) antibody images

  • ab111889 at 1/50 dilution staining BLK in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized peptide.

References for Anti-BLK (phospho Y501) antibody (ab111889)

ab111889 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your interest in ab111889. To our knowledge, ab111889 has not been tested in WB. Please let me know if you have any questions about this offer and I would be happy to help you further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"