Overview

  • Product nameAnti-BLMH antibody
    See all BLMH primary antibodies
  • Description
    Mouse monoclonal to BLMH
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment: NMNKAERLTF GESLMTHAMT FTAVSEKDDQ DGAFTKWRVE NSWGEDHGHK GYLCMTDEWF SEYVYEVVVD RKHVPEEVLA VLEQEPIILP AWDPMGALA, corresponding to amino acids 356-454 of human BLMH (NP_000377) with a 26 kDa tag

  • Positive control
    • K562 cell lysate; recombinant tagged fragment of human BLMH (the immunogen).

Properties

Applications

Our Abpromise guarantee covers the use of ab77111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged BLMH is approximately 0.3ng/ml when used as a capture antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 53 kDa.

Target

Anti-BLMH antibody images

  • Anti-BLMH antibody (ab77111) at 1/500 dilution + recombinant fragment of human BLMH (the immunogen) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

    Predicted band size : 53 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-BLMH antibody (ab77111) at 1/500 dilution + K562 cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

References for Anti-BLMH antibody (ab77111)

This product has been referenced in:
  • Baglo Y  et al. Enhanced efficacy of bleomycin in bladder cancer cells by photochemical internalization. Biomed Res Int 2014:921296 (2014). WB . Read more (PubMed: 25101299) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"