Anti-BLNK (phospho Y84) antibody (ab79320)

Overview

  • Product nameAnti-BLNK (phospho Y84) antibody
    See all BLNK primary antibodies
  • Description
    Rabbit polyclonal to BLNK (phospho Y84)
  • Specificityab79320 detects endogenous levels of BLNK only when phosphorylated at tyrosine 84.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    The antiserum was produced against synthesized phosphopeptide derived from human BLNK around the phosphorylation site of tyrosine 84 (EMYPVM).

  • Positive control
    • Human brain tissue and extracts from K562 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+)150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 51 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • FunctionFunctions as a central linker protein that bridges kinases associated with the B-cell receptor (BCR) with a multitude of signaling pathways, regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition (By similarity). Plays an important role in BCR-induced B-cell apoptosis.
  • Tissue specificityExpressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
  • Involvement in diseaseDefects in BLNK are the cause of agammaglobulinemia type 4 (AGM4) [MIM:613502]. It is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.
  • Sequence similaritiesContains 1 SH2 domain.
  • Post-translational
    modifications
    Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca(2+) and MAPK signaling pathways.
  • Cellular localizationCytoplasm. Cell membrane. BCR activation results in the translocation to membrane fraction.
  • Information by UniProt
  • Database links
  • Alternative names
    • AGM4 antibody
    • B cell adapter containing SH2 domain protein antibody
    • B cell adapter containing Src homology 2 domain protein antibody
    • B cell adaptor containing SH2 domain antibody
    • B cell linker antibody
    • B cell linker protein antibody
    • B cell-specific adaptor protein antibody
    • B-cell activation antibody
    • B-cell adapter containing a SH2 domain protein antibody
    • B-cell adapter containing a Src homology 2 domain protein antibody
    • B-cell linker protein antibody
    • BASH antibody
    • Bca antibody
    • BLNK antibody
    • BLNK s antibody
    • BLNK_HUMAN antibody
    • Cytoplasmic adapter protein antibody
    • Ly 57 antibody
    • Ly57 antibody
    • Lymphocyte antigen 57 antibody
    • Lyw 57 antibody
    • Lyw57 antibody
    • MGC111051 antibody
    • SH2 domain-containing leukocyte protein, 65-KD antibody
    • SLP 65 antibody
    • SLP-65 antibody
    • SLP65 antibody
    • Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody
    • Src homology 2 domain containing leukocyte protein of 65 kDa antibody
    • Src homology 2 domain-containing leukocyte protein of 65 kDa antibody
    see all

Anti-BLNK (phospho Y84) antibody images

  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using BLNK (Phospho-Tyr84) antibody at 1/50 dilution in the presence and absence of immunizing phosphopeptide
  • All lanes : Anti-BLNK (phospho Y84) antibody (ab79320) at 1/500 dilution

    Lane 1 : Extracts from K562 cells at 30ug of total protein, treated with starved (24 hours)
    Lane 2 : Extracts from K562 cells at 30ug of total protein, treated with starved (24 hours) + 10ug of immunizing phosphopeptide


    Predicted band size : 51 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.

References for Anti-BLNK (phospho Y84) antibody (ab79320)

ab79320 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The BLNK antibody ab79320 recognizes the sequence EMYPVM. This correlates to the phosphorylated tyrosine 84 in the UniProt sequence Q8WV28 and the NCBI sequenceNP_037446.1. Our system automatically sets the "Predicted ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"